XB-CLONE-4354399
Clone Name: IMAGE:7026664
|
Gene:
ckm
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF783015
[+]
taattaatcccccccccccccccccggggtcaccctgaagtttgctccgtctctaaagcaggtctagcccgatcttccaacatgcctttcggtaacaccc
ataataactggaaattgaattattcctctgaggaggaatatcctgacctcaccaagcacaacaatcatatggccaaggccctgtctgctgacatctacaa
gaagctcagggacaagcagacaccaagtggcttcaccttggatgatgtcattcagactggagttgacaacccaggccatcctttcatcatgactgtgggc
tgcgtagcaggggatgaggaatcctatgaggtgttcaaggacctttttgatcccattattgaggatcgtcacggtggttacaagccaactgacaaacaca
agacagacctcaactttgccaaccttaagggaggtgatgaccttgacccaaactatgtgttgagcagccgtgtaaggactggccgtagcattaagggata
tgccctgcccccccactgcagccgtggggagcgtcctgccattgaaaaacactccacaaagctctcaacagtctgtctggggaattcaaagggaaaaact
acccttctcaaggatatgtcagaaggcaaagcagcaccaactgaattgagaccaccttcctgtttgacaaaacaatggcccccctgcttctggccctttg
ggaatgggccgtggaagggcctgaaggcccctgggattctgggcacaaaggaaaacaaaaactttcctggggggggggtaaatggaaaaaaaaccttttg
gaaaaatcattttccctggccgaaaaggaaggaaaacacttgaaaaaaaaattttttaaaaaacccttttctgggaggggaactttccaaaaaattttaa
aaaaaattttttttaaaaaaaatgcgaaac
3' EST: CF783014
[+]tttttttttttttttggttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttt
tttttttttttttttttttttttttttttaaaaggaggggagaggggggggtgggcgccccccaacaaggggggggggggacaaaaaaaggggggggggg
ggggggggggggaaaaaaaaaaacctcccgaaaaaaaaaaagcccccccccccccccggaggggaaaaaaaaacgtttgttgggggggggggggcccccc
ccccccaggggagaaaaacccccccccccccccaaaaattaggacccccccccggggggggggtttttggggccccaaaaaaaaaggaggggggcgcccc
cccccccgcccaagggaaaaggaagggggggtaaaaaacacccccacccccccccacccagacagcgcataatgggggggccagaaaaaaaaaggagggg
gggccgcccgcacggaggaggggccccgccccaaaagagaggagagggcacacaaaccaccaccacagtggggggggggggggggccacaaagagaaagg
ggcccccaccgacaaggggaggggggggagaaaaaagaacgccccccccccccggggggggggggagccaaaacaggccagaggggcgccgacaccgcga
gaaaagaaaagagacggtcggagagcatgaaaccaaagaagagggggggggggatacgcaagaagcgagggggggaggcaaaatcagaagaaaagacgac
accaccccccgcgacaagggagaggggaacgcactgaatagatgcggggcgaggcgaaagaaaaagaaaacac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: