XB-CLONE-4354396
Clone Name: IMAGE:7026663
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Gene:
aldoc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783017 [+]
taattaatccccccccccccctaccagatccagtgcgccgctctactcttagcgcctagcatcacccgaattcacgaaaatgactcaccagtacccagct
ctctctgctgagcagaagaaggaattggcagacatcgctcaaaagattgtggcacctgggaagggcatcttggctgctgatgaatccgtggggagcatgg
ccaaacgactaagtggaattggtgtggataatatcgaggagaacaggagactgtaccgtcagttgctcttcaagggtgacgagcgtatgcaggaatgtat
tggtggtgtcatcttcttccatgagactatgtaccaacagggtgactgcggcaccaattttgtcaagatgataaaggacaaaggaattgttgttgggatt
aaggttgataaaggggtggtccctcttgctggcaccaatggagaaactactactcagggtttggatggcttgtcagaacgctgtgcacagtacaagaagg
atggagctgattttgccaaatggcgttctgtgctgaagatcagtgagaccactccctctgcccttgctattctggaaaatgctaatgtgcttgcccgcta
tgccagtatctgccagcagaatggcattgttccaattgtggagcctgaaatccttcctgatggagacccatgacctgaacgctgccagtatgtaactgag
aaggtgctcgctgcagtgtacaaggctctcagtgatcatcatgtatatttggaaggcactttattgaaaaccaatatggtaactgctgggcacgcctggc
ccaccaaatacactggcaaaaggagattgccatggncaacggtaaactgcn
3' EST: CF783016 [+]tttttttttttttttcaatatataatttattttcatactatttcgtacatcagtttcagacagagacagacaacacactagtttcacaagatacacagcc
attcattagttgcccttcccaatagctctcctttcagttctaatctcattttacttgccaaagacctaacaacatgggaaggacctgcttttagtggaat
aggaagcgcaacacttctcacactacctacctatagaagtaccgcaaagtgcaagacagaacagtgtcatggattgaatgaacagtagatatttgggtgc
agcttgtttttgtgcacgagaataataaaactggatatataccaccgtagaattatctccaactgcaccatatgttaatatttcctgtccaacaatggac
acactattgacctatttagaaggattaccaacaatctgcactcctcttattatggctgtaaagcaaaatctttcacaaaaataaatgttttgttagggaa
atatactacaaccaggtacattatgctgcaagggtgggtgaagctgattcattcactcacaaactccctccatttactcaagatccctttaaccacctta
ggttaggggaaaaaaacgggggaagaaaaagggttgggacaattcccgggctttggggtgcgggggatgtcccaaaggggaggccttctttacaggaaaa
aaaaaaaaagggtctcttggagggggaggggggccccggggggaggggggaaaaaccttttttatggggtgggggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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