XB-CLONE-4354393
Clone Name: IMAGE:7026662
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Gene:
prmt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783019 [+]
taattaatcccccccccccccggcagttctctcgtacaaagccggtggcgtggctttgagtttcttttttagattcgtgagagagactgacaaacatggc
tgaagcgagcacctgcaacatggaggtttcttgtactcagcctgagagcagtgtgaagccaaatgcggaggacatgacatcaaaagattactactttgac
tcgtatgctcactttggcatccatgaggagatgttaaaagatgaagttcgaacgctgacatatcgtaactcaatgtttcacaacagacatctatttaaag
ataaagtagtcttggatgttgggagtggcacgggtattctctgcatgtttgcagcaaaggctggtgccaagaaagttattgggattgagtgctcaagtat
atctgactatgccataaaaatcgtgaaggcaaataaactagatcatgtggttacaatcatcaaaggcaaagtagaagaagtggagctcccagtagaaaaa
agtggacatcataatcagtgaatggatgggatattgtctgttctatgaatcaatgctgaacactgttatctatgcacggggataaagggctgactcccga
tggggttaaatatttccagaatcgtgctaccctggtatgttaactgcctattgaggggatagacaatatcaaagacctaccaaaatttccctgggtgggg
gaaaaccgtttatggggctttgaaaatggccatggcctcccagggatggtaacccctctcaaggaagccccccgggttgaaggtttgtttgaatcccaaa
aaaactgggtcccctataggccttggccctttaaaaagggaaagttggaaatttttatcctggcccaaaagttggaacgaaacctgaacctctttaaccn
tcccccatttcttgcccgtgcaagggtgaaaccgggaaagggttatacatttccatggcccccaagattggcctttattttcagaccttgtggaat
3' EST: CF783018 [+]tttttttttttttttcttttcacagttcactttttaatttcattatgaatgcaaccatataaaaaccataagttatgaaacagtcacaatgggaagcttg
aaggatcaataaaacaaatgaagggcctttccataccaaggtctgagaggacttacaaaaacagtacaaaacacccaagccaagtgagcaacccttttgt
tcctcaataggatgggtgcctgctcctcataagggctagacaagggatactggtgataccatacacattgcaacaggacagaaaacccaccctgatagtg
tccattagttaaaaaaaaaaaaaaaaagccctgcaaaaaaaagtgaaaaggggaatgcacactttggagcttaacactttgctgccagcaggggctgaat
acttttgcagtgtatccccctttaggccactcagtaatcaagtaatgggtttcctattgttaaaatagcctgcaattctagcaattcattgactgaatat
actaaaaacaaaaaggaatgcctcttgggatagacctgctaaaggcaggaaaacttagggggctcggaaactgaacaaatggtttgaatccattatggat
ctttatttacatgatgaaacccagccaaggagctggatcaatactctagcccttaatggccacagtggaagacaagatcagggccttttggtttcaccaa
ggagctcttgcactctacactgaacctgggccttctaaaaagttccccattttgtggctaccaacaagggttaaaaacttttgtagaatatgtaaaataa
aacccccccccctaaaggttaaaacatgaaagccttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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