XB-CLONE-4354388
Clone Name: IMAGE:7026660
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Gene:
exosc8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783022
[+]
taattaatccccccccccccccgataggcaacacgtgtggctgagccggggctgcccattggcagacagggagccggttgtgcaagttgggtacaaggag
gtcacagggggctccgcgctgaggcctgcaacatggcggctggtttcaagacggtggaacctttagagtattacagaaaattcctgaaggaggcttgtcg
gccggacggtcgagaactcactgagtttaggacaacgaccataaatgttggttccattaccacagcggatggttcagctttagtgaagcttgggaattgc
actgtactatgtggggttaaagcggagtttacagctcctcccgcggatgcccccaataaaggatatgttgttccaaatgtggatcttacacccttgtgct
cctctcagtttcgacctggtcccccaggggaagaggctcaagttgccagtcagttcatagcagatgttattgacaattcccagatgctcctgaaagaaga
cttgtgcatcgcacatggcaagctagcgtgggttctgtactgtgatctcatatgtctcgattacgatggaaaccttcttggaaacttggcatgtgttggc
tttgggttgcttgctttacagaattgtaagaattgcccatcctgttaaaaataaaccgaagaagactgggtttaaccataagttcgattttgaaaaaaca
aaaacttgcctcttgaaaaaatcctgagaaaaaacccccggttggcaaacatcctttttgccaggaaatttggaatagaaaaaattttcctttccggggg
ggaatcccccaccggggggaaataaaagaagggggtttgggatcaccacccggaactatctccaacctggtgagtggaactcgaatgaaaaaattgaaaa
acgggtgttagtgttggccccattctcagaataaccccagtttgtgggaaaattgtccctttccacaccaccatgaagaaaggatctccaccacagtagg
ggctat
3' EST: CF783021
[+]tttttttttttttttagccatggtaatgtttatttagtacaaaaggtgtgcaatcactgtaacaaatacaactttttacatggtcatttttaaaatgaat
atgcctacttattctgacttgacactggccagcgcctcttgcagaaggttcgtcacctctttgtgtctcgtgatggctcgactgatgcactcctggagct
tctctgctgcaagggcactcccacctggttttcgagtggcacataacctttcatcttcatcagtcacaacagtgagtagtccgctggccaagccctcctc
ttcccctgtgggatccaccagaaggaatgtgtcatcaaatactgcaaatgatgttgcaacggggtgtttcaggattttcagagcatgttttgttttcaaa
ttgacttctgctaaaccagtctcctcgtttatttttacagatggcaatcttacattctgtaaagcagcaaccaaagcacacatgcaagtatcaagaaagt
ttccatcgtaatcgagacatatgaaatcacattacagaacccacgctagcttgccatgtgcgaagcacaaagtcttctttcaggagcatctgggaaattg
taataaacatctgctataaactggactgggcaacttgagcctcttcccctggggggaccaggtccaaactgaaaggagcacaaggggtgtaagatccact
tttggaaacaacatattcttttattggggggcttcccccggaaggaacctgaaaatcccccttttaacccacacataataaaggtggcaattccccaagc
tttccctaaagc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: