XB-CLONE-4354383
Clone Name: IMAGE:7026658
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Gene:
zmat2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077058 [+]
CCGTAGGCCGGGCAAGATGGCGTCGGGAAGTGGGTCGAAAAATGCAGACTTCCGTCGGAAGTGGGACAAAGATGAGTATGAGCAACTTGCACAGAAGCGA
ATTACAGAAGAAAGAGAAAAGAAAGATGGCAAACCTTTACAGCCCATTAAGAGGGAATTACTCCGACACAGAGACTATAAGGTGGATCTGGAGTCCAAGC
TGGGAAAGACCATTGTCATTACAAAGACCACTCCACAGTCTGAAATGGGAGGCTACTATTGCAATGTCTGCGACTGTGTGGTGAAAGATTCAATCAACTT
CTTGGACCATATCAATGGCAAAAAACATCAAAGGAACTTGGGAATGTCAATGAGAGTAGAGCGGTCAACACTCGATCAAGTCAAGAAGAGATTTGAAGTT
AATAAGAAGAAAATGGAAGAAAAGCAAAAGGACTACGACTTTGAAGAGCGAATGAAAGAGCTTCGGGAAGAGGAAGAAAAAGCACGAGCGTACAAAAAAG
AGAAACAGCGGGAGAAAAAACGTAAAGCAGAAGAAGACCTTGGATTTGAAGAGGACGATGAAATGGCTGCTGTCATGGGTTTCTCAGGCTTTGGGTCATC
AAAAAAGGGTCACTAGTTCTGCAGAACCATGTGGGCAGCTTTTTTTTTTTTGTATTTCCTAATGAAACCTATCTTTTTATATAGACAAAGCTTTTTTTTT
TATAACGATATACCACTTTGACACTGGCTAACCACAGGGAATGATGGGAGTATGCACCCAGCAGCCAAAACACACAATGACTATTACTGTGAAACTTTTT
TTTTATTTAAAGATCTGCAAACTTTAATGCTGTGGGCTATTGGTCAGACTTTGCCAGTGGCAAGGTGCGCTTTCTTTTTTCTTCCTGTTTGAAATGTGTT
ACATGTATAGTAGGACAAATCTGCTTGTGAGGCAGCCCTTTCACTCTGATCCAGTAGATTTTCATTGTAGTAAAGTAAAACCTATTGCTTTTAATAATTA
AAAAAAAAAAAAAAA
5' EST: CF783025 [+]taattaatcccccccccccccccgtaggccgggcaagatggcgtcgggaagtgggtcgaaaaatgcagacttccgtcggaagtgggacaaagatgagtat
gagcaacttgcacagaagcgaattacagaagaaagagaaaagaaagatggcaaacctttacagcccattaagagggaattactccgacacagagactata
aggtggatctggagtccaagctgggaaagaccattgtcattacaaagaccactccacagtctgaaatgggaggctactattgcaatgtctgcgactgtgt
ggtgaaagattcaatcaacttcttggaccatatcaatggcaaaaaacatcaaaggaacttgggaatgtcaatgagagtagagcggtcaacactcgatcaa
gtcaagaagagatttgaagttaataagaagaaaatggaagaaaagcaaaaggactacgactttgaagagcgaatgaaagagcttcgggaagaggaagaaa
aagcacgagcgtacaaaaaagagaaacagcgggagaaaaaacgtaaagcagaagaagaccttggatttgaagaggacgatgaaatggctgctgtcatggg
tttctcaggctttgggtcatcaaaaaagggtcactagttctgcagaaccatgtgggcagcttttttttttttgtatttcctaatgaaacctatcttttta
tatagacaaagcttttttttttataacgatataccactttgacactgggctaacacagggaatgatgggagtatgcacccagcagccaaaacacacaatg
actattactgtgaaactttttttttaatttaaagatctgcaaactttaatggctgtggggctaattggtccaaattttgccagtgggcaaggtgcgcttt
t
3' EST: CF783024 [+]tttttttttttttttaattattaaaagcaataggttttactttactacaatgaaaatctactggatcagagtgaaagggctgcctcacaagcagatttgt
cctactatacatgtaacacatttcaaacaggaagaaaaaagaaagcgcaccttgccactggcaaagtctgaccaatagcccacagcattaaagtttgcag
atctttaaataaaaaaaaagtttcacagtaatagtcattgtgtgttttggctgctgggtgcatactcccatcattccctgtggttagccagtgtcaaagt
ggtatatcgttataaaaaaaaaagctttgtctatataaaaagataggtttcattaggaaatacaaaaaaaaaaaagctgcccacatggttctgcagaact
agtgacccttttttgatgacccaaagcctgagaaacccatgacagcagccatttcatcgtcctcttcaaatccaaggtcttcttctgctttacgtttttt
ctcccgctgtttctcttttttgtacgctcgtgctttttcttcctcttcccgaagctctttcattcgctcttcaaagtcgtagtccttttgcttttcttcc
attttcttcttattaacttcaaatctcttcttgacttgatcgagtgtttgacgctctactctcattgacatccccagttccctttgatgtttttttgccc
ttgaatatggtcccaagaagtttgattggaatcttttcaccacacaagtcgcaaaaaatttgcaaataat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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