XB-CLONE-4354380
Clone Name: IMAGE:7026657
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Gene:
extl2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080459 [+]
GCTACTGTGTGGTCGGCAGGGCAGCTGCAGCCTGCAGGGGCCTCAATGACAGAGCTTCTCTCCTTAAAAGGATATGAGGTGGTTGAGGAGGATAGTGAAG
TTAAGGATGCTACGCATGTCCTGTGTTGTCCTAGTCCTCCTCCTTCTCTTTCTGGGGGCTCTGACTGCATTACTTCCTGCTACAAATGAAGATGCTGTGG
TGGGTGTCCGGCGTGGGATACCCCTCTCTGCTGTGTCCCCCAAAGACGTCTTTACACTCATCATGCAGACCTATAACAGGACAGACCTACTGCTGAAGAT
GCTCAACCATTATCAAGCCATGCCAGGCCTTAGCCATGTGATAGTGGTATGGAATAATGTTGGCCAGGATACCCCTCAGGAGCTGTGGGAATCTTTTGGT
CCTCATCCAGTACCTGTCACCTTCAAAAAACAGAAGGTCAATCTCATGAGGAATAGACTGCAGAGCTTCCCAGAGATTCAAACCCAGGCTGTTTTAATGA
TGGATGATGACACCCTAGTCAGCGCTTATGATATTTCATTTGCCTTCTCTGTCTGGCAGCAATTTCCCGATCGCATTGTAGGATTTGTGCCCCGGAAGCA
TGTGTCCTCCCCCTCTGGTATTTATAGCTACGGTAGCTTTGAATTAAAGGCACCACATACAGAAACTGGAGATATGTACTCTATGATACTCATTGGAGCT
GCCTTTTTTCACTCGGACTATCTGCGTCTCTTTGAGCAGCTGCCTGCCTCCGTCCATAACATGATAGATCAAACACAGAACTGTGATGATATCGCTATGA
ATTTTATGGTGGCCAATCACTTGGGGAAAGCCTCTGGTGTACTTGTCAAACCCACAGATATGAGGAATCTGGAAAAAGAAGCAGGAAGTGGGTATACAGG
CATGTGGCACCGAGCTGAACATCTCCTGCAGAGATCCTTTTGTCTGAACAAGCTGGCCGAGATTTACAGCACAATGCCCTTGAAATATTCCAGCATTATG
ATCTCACAGTTTGGCTTCCCCAACTATGCCAACCACAAATCTAAGATATAATGTGTAATATTCAAAACATTATTGCTCCCATGTGGGTCTTACAATTTTT
GGACTTTTTGGGATCAAAATATACTAATACAGAGACTTGTACAAACCTGAACTCTTATTGGTTTACTCAGATAGAGATCAAATGTGTTGAGCTGTTTTAG
GAGGATTCTTGCTTTTTGTTTTTGTGTGTTCAGTAAGATCAAGAAATAGAATGTTAATTTTTGGGATTTTGCTTGTTTCCTAAGTTCTCTTGGTGCAAAA
ATCAGTTTTTTTAGGGATAGCTAAACAACACTGCAAGACTCTCTTCCAGTTAACTAAAACAAATAAAACGTTTTTATTTCAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF783027 [+]taattaatcccccccccccccgctactgtgtggtcggcagggcagctgcagcctgcaggggcctcaatgacagagcttctctccttaaaaggatatgagg
tggttgaggaggatagtgaagttaaggatgctacgcatgtcctgtgttgtcctagtcctcctccttctctttctgggggctctgactgcattacttcctg
ctacaaatgaagatgctgtggtgggtgtccggcgtgggatacccctctctgctgtgtcccccaaagacgtctttacactcatcatgcagacctataacag
gacagacctactgctgaagatgctcaaccattatcaagccatgccaggccttagccatgtgatagtggtatggaataatgttggccaggatacccctcag
gagctgtgggaatcttttggtcctcatccagtacctgtcaccttcaaaaaacagaaggtcaatctcatgaggaatagactgcagagcttcccagagattc
aaacccaggctgttttaatgatggatgatgacaccctagtcagcgcttatgatatttcatttgccttctctgtctggcagcaatttcccgatcgcattgt
aggatttgtgccccggaagcatgtgtcctccccctctggtatttatagctacggtagctttgaattaaaggcaccacatacagaaactggagatatgtac
tctatgatactcattggagctgccttttttactcggactatctgcgtctctttgagcagctgcctgcctccgtccctaacctgatagatcaaaccccgaa
ctgggatgatatcgctatggatttttattggtggccac
3' EST: CF783026 [+]tttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttgaaaaaaaaaccttttttttttttttt
ttaaccgggaaaaaaccccgggggggttttttccccccccccaaaaaaacggttttttccccccaaaaactttgggaaacccccaaacccccaaaaataa
aattttttttttggggttttcggggccccccaaaaaaaaaaggggaaacccccccaaaaaccccccccccctttttttcccttccggaaaaacccaaaaa
aatttcggggtgggccaacccccggtaaaaaaaattttggtcccccaaaaccccaaaatttggaaaccccccggggggccaaaagggtttggaaaatccc
ccctttatcctaaaatttggggggggcaaatggggggaaccccaccggggaaaccaaagggggggaaattttcggggggttgggggggaaaaccccgccc
cccttgttaaaaaaaaaggttccccccgggaaagtttcccccgggggccccaggccggaaaccccccccccggctttttttcccaaaacccccaaaaggg
gggggtggaaaaaacccccaaaggtttcccccagggggggggccccccaaaattttaaggggaatcccccccagttgggggttgggacaaccccggtagg
ggggggggggggccccccctcaaaaaaccccaaaatccccgggggaaaaaggggcccccccagggggttcccaaaggcaattccccccccgtttttggaa
gggggggggccctttataa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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