XB-CLONE-4354374
Clone Name: IMAGE:7026655
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Gene:
krt12.5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783031 [+]
taattaatcccccccccccccgacattctgttttgtgcagactgagttgtcaggcattctcttccaataacagtcaccatgtctctgcgttctagctctt
cttattctgtacagagcaaaagtgtctctggaggtggtggttttggcagcggctatggggctagctttggtggaggaagctttagtggaggaagctatgg
tggcagcagctttggtggtggctttggagcaggctttggtggtggtgcagcttctgggttttccgctagttccgcaggtggttttggagcagcatccgct
agttccagcttcagcagctttggcggaagcgacaagcaaaccatgcagaacctcaatgaccgtcttgctgcctaccttgagaaagtgagagccttggaag
ctgccaatgctgacttggaactgaagatccgcgagtggtatgagaagcaaagaacctctggcattggagcaggggctaaggatttcagcaaatactttga
tatcatcactgatttgaggagcaagatcctttttgccaccattgataactcaagagttgttctgcaaattgacaatgccaaacttgctgctgatgacttc
cgtctgaagtttgagaatgagctggctcttcgccagagcgtggaggctgatatcaatggcctgcgtaaagtcctggatgaactgaccctggctagaggtg
acctggaaatgcagattgaaaaacttgacttgaaaaacttggcccacctgaaaaaaaaacctggaaaaagaaaatgagccttggctaaaaacaattgctg
cccggccaaagtgactgtaaaaaattggacacctgcttccaggcccaaaatcttgaaacaacatttctggaaataaattggaaaaccgccaactatttaa
aacatctgggcttgaaaataaaacaaat
3' EST: CF783030 [+]tttttttttttttttggcttggaaagctttattgcatcttgaaatcaacagaaatttccattttgtgagtgaagatttcagagatgaatctttatttgac
gttctcttcgacttcctcaactcttgatgagataacctttccatcgaccacttcttccacaatggtctttacttttctggtttttgatggatcttttttg
gagctggaagatgattgtgatgtgattacttgcactgatgaactggaagaactcaattctccctccagcaggcgcctgtatgtttcaatctccatctcca
gcctggtcttaatgtcaaggagctgcttgtactccatgttctgacgttccatgtcggacctgatctgcagaagttgttcctctaggcttctgatactgag
ctggatggtttgaagctgggcaccatagcggccatctgtttctgccagagagtcttcaagagattttttcattgccagttgagattgcaattcaatttcc
aagctctgaaggctgcgtctgaggtcggatatttcgctcttgcttgactgcactagttccacgcctgttaaaatctctttcttcagctcaccactcttct
ggttgaaccacagctctgcatcccttctgttcttctcagccagtgtttcatagtcggctctcatgttattcagaatgttggtcagatctgtgcctggagc
agcgtccatttctacagtcacttggccggcagcactgctcttagcaatgctcatctcctcttcatggttcttcttcaggtaggcccagctcttcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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