XB-CLONE-4354371
Clone Name: IMAGE:7026654
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Gene:
hes4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783033 [+]
taattaatcccccccccccccagagtgagatagaatccaggctgtgggcacagaatccagccaagcctgacgcacagcacacgcctctgcactgctgtta
gcagcacatccgcagcactaccccctattaggatacaacatcccctcctgactgcaccagtgcccgggaactgaacatgcctgccgacagcatggagaag
cctaccgcatccccaattgcaggggcaccggcgagctcagcacagactccggataaacccaagagtgccagtgagcacagaaagtcatccaaacccatca
tggagaagcgcaggagagcgcggatcaatgagagcctgggccagctaaagaccctcattctggatgcgctgaaaaaagatagctccagacattctaaact
ggaaaaggccgatatcctggaaatgacagtcaagcaccttcggaacctgcagcgagtgcaaatgacagccgctcttacagccgatccctcggtactaggg
aagtacagagccggatttaacgagtgcatgaacgaagtcacccgattcctctccacctgtgagggcgtcaatactgaagtgaggaccaggcttctcggtc
acctctcaagctgcctgggacagatagtagccatgaattaccagcaacccccctccagccagcaaccactgcacgtacagctttccttccagcacccctg
ccccctgtgcccatgcccttgcaaaggtgaacccagcaagaggccaatctctccccaaattattcccaggggaggaattcccagcttgggggccaagccc
accgaacggggaagttttggcctttctcttnaattccccaaaacaccgcctttaataccaaccaagccccccgggacccttgtttaatccccctcctaat
tagggccaacaacccgaacggtggaaacttccccctggc
3' EST: CF783032 [+]tttttttttttttttcaaatgaaaagagtttattcaataaaactcctgcatgttcggaaagtttttgacacaaaaactaacacaataaactttggcatac
acatttttgtacgcaattgtacaaaactaaaaaacatctcagaaaaaagagctttgttacaaaaatgggtcatctatacaagtttgttttatttattaaa
acaataaaaaagtgaaatccccaaatttggaaataaatagcattttgtgcagattgtctttaaggcagggtcccatacaacggaaccaatatatacacag
tctgatggctgttcgcgttatcaccatggtctccacacggactcagcgctgtccgccccggtgctgccccctaggggactgacagcttgtgccatgtgag
gcatcttgtggccgaaggtggtgagtccctgtactggaaattgactctggggaccccccggcgatgtcacgttggtgttggcatataaagggataacagg
gtccccgggcctggtggtataaaactggggttggggaataaggaaaacaaactgcccgtcggtggctggaacccaactgaaaccctccctggaaaaactt
tgggaaaaaaaggcctctgcctggggttcacccttgacaggcatggggcacaggggacaggggtgtctggaaagaaatctcgttcttgccactggtttcc
tggctggaaaggggggtctcctgggaatatcatggggtaataaattggccccagggcatctttgaaaagggtgaacccaaaaaaccccgggccccccacc
ttcaataatataacccccctcaaaagagtggaaaagan
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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