XB-CLONE-4354365
Clone Name: IMAGE:7026652
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783037 [+]
taattaatcccccccccccccgcttcctcaccgggtctgcggcgagttttaagtgtccaccgccaaacatctaacaatccacaatgggaaaggaaaagac
tcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaactggacatcttatctacaaatgtggtggtatcgacaagagaaccatc
gaaaagttcgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggacaaactgaaggccgagcgtgaacgtggtatcacca
ttgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccaggacacagagacttcatcaagaacatgatcactggtac
ctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtatctcaaagaacggacaaactcgtgagcatgccctcctt
gcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaacccccatacagccagaaaagatatgaggaaatcgttaagg
aagtcagcacatacatcaagaagattggttacaaccctgatactgttgcctttgtacctatttcttggatggaacggtgaaaacatgcttgagcccagcc
gccaatatgccttggtttaaggggggggaaaaactcacntaaaaaagggattctggcagcggaaactacccctgcctggaaaccctttgaacgggccttt
ttgccccccaagttccccccaactggaaaaaacccttcttgcggtcctggcccctgggcagagaatgttcttacaaaaatatgggga
3' EST: CF783036 [+]tttttttttttttttcttttaacttttttttaatggtctcaatttggtgacagattttggtcaagttgcttccatttagaaattactggatttaaaacac
taataacttaaaaactgccacaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgactat
taaactctgatggccaattgatagaaagagttctgagaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcctttc
tgtgctttctgagcagactttgtgactttgccagctccagcagccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacgga
cagcaaaacgaccaagaggggggtagtcagagaagctctccacgcacataggctttcctgggatcatgtcaacaatggcagcatcaccagacttcaggaa
cttgggattgtcttccagtttcttaccaaaacggggatcaatctttttcctttcgctcaacaaacttgcaagcaatggtgacctgggtggcaatccaacc
acgggggcatatccagcacccaatctggcctggggtggtttcaggaagaaaacctggtgcgggaaaagggaccacccttccacttgggggggacatttct
tgcgggacacccacaaacctttgccaaacaagggaacgtcctttccaaaaaaaacgtttttttccccttataagcccaaaatatggtccaccg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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