XB-CLONE-4354359
Clone Name: IMAGE:7026650
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Gene:
banf1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783041
[+]
tacttaatccccccccccccctagattttgagctttcgggtcagttggcgggaagacgtgaaaagagtgaaaatgtcatcaacatcacagaagcacagag
actttgtggcagagccaatgggagaaaagccggtccaatgtctggcgggtattggcgaaacactggggcataggcttgaaaaaaaagggtttgataaggc
ttatgtcttgtagggtcagtttctcgttttgaagaaggatgaagagcttttcaaggataggctgaaggatacttgttccgccaatgccaaacagactaaa
gattgctttggctgcctaaaagattggtgtgatgcctttttgtaaaatagtatctctcctcgttgtgatgccatttgaaaagagaggcaaaaataaaacc
gcctaaatttagaagaaaaaggcaacaaaaatttgacagaatacgtaattatgatcggaagggcaaataaaaacttgaaccacgttagggaagaaaaagt
aagtgcgggctgaagaggaagggaggccctagtgggggctgagtgatgataggttttggatgggtgaaactgagggggtggaagaaggggctgtgggttt
aagggtttcggtctacaataaacgtaataatttttatatataagatgtcgacgccccacatcattagataaatttataatccgttattttgttaaaatta
cgccttctgttgttatactccatttatgagagggattggagagtaagtgtgagaacaaccaatcaaagggtgtgggtggaataacaatctgtttttnggc
tgtatgttattaaaatagggggcggtctatatgtagttgtacanttggtgatcacctgtgtgaaatgtatgcacgtagaaacttaagaataggaaggttg
ataagtcgtcggaccacagttatacaatctgtcttatatattaatggatgtcttaaagcccaaaccaaattatgtcaacacacaaataaaatccttgaaa
atataattaatgttcaaacgagtagataaagtggaaacaaaggagacagagccctcgcggtggggtggtagtgactgacgcgcggttggccgtattacaa
acatagcaacccatatgtctgggtgtggcgatattacgatcatccttctggtgaggcagggcgcctcggcctcgaagcgaaaagacataatgattagtat
aaaaataaatacaataagatacaagataaagcatgattatttagattagttaggaacga
3' EST: CF783040
[+]tttttttttttttttccttttttttttttttttttttttttttttttttttccaaaggcaaaaatttttttttgaaaaaaaaaaaaaaccaagcccggaa
aaatactgggaacaaagggacggggtaaaagggaaagtttttcaccaggaaaagtttttggaaaattcctctaaaaaacacctgggaaaaaccggcgcca
cgggcggcaatacccgggaaacaggggtttgccaaccccccaaattattaccggaaaaaatcccccacagaaatatccttacgagaaaccccactaagcc
ggaacacccgggtacatccccccagggcaaaaaaattgataaaaaagggtcctatgccccccttaaaaaccctccgggggggaaacaacgggcctttttc
ccgggccacccggggggaaaggaaaacccccccttgaccccccaggggaaacccaaggggggtttttgggccaaaaaacccttataaaaaaaaaaaaagg
ggggccaaaaaatcccccccaaaggcccaaattcccccccaattttttaaaaaaaaaaccccccaaaaaggggggaagggggaaaacccccaaaatgtgt
ttaggggggagttaaaacaccccgcaaaccccgggggtgggcgcttttttttaggggggggggaattttttaaaaagcaataactatttaaaaaaaaccc
gtgggccccccccggggaaaaaatttccccccccctaaaaaaaaatcttaaaagcggggtataccccccccaaaaaggcccccacaaaaaaattttttgc
gcggggcacccgagggggccccccttatattaggaggcgcgccctcgccaaaaaaacgcgagaaacctccccccccccgttgggggggggggagcaatcc
aacaaaatg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: