XB-CLONE-4354357
Clone Name: IMAGE:7026649
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Gene:
uqcrc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723009 [+]
taattaatcccccccccccccgcctgtcctgtgctaatgtcagcgggaagatggcggcttcagtgtgcagagccggtagctcagtagggaaagcgctgtt
acgggcccgctcgccggcgttgctgaacccccagaggaatggcagcgcggtgccctatacccgggcagtacagaacatccccgagacccagatctccagc
ctggccaatgggctacgtgttgcctccgaggagtctggccaagccacctgcactgtgggcgtttggatcggaactggcagtcgctacgagaacgataaaa
acaacggcgctggctatttcctagaacatctggcatttaagggaacaaagaaacgcccccaggcaccattggaacaggaagtggagagcttgggcgccca
cctaaatgcctactccactcgcgagcagactgctatatacatcagagccacagtcccatggacctgcccaaaggctgtgtgaaatattgggccttacttt
ggtgcattaatttgcagcccgggaaggactcctcagaattagagaaaggagccgggccccgtgaaaactcgccgggaaaaatgcctggcaaattataact
ccctaagccttagaaggtaatgtggtgtgttttggaactaaacctttcaattggctcaatgggccgttattcacaagaggcaaccgcccctcttgggtgg
ccgcccctcttgtgagtgtatttgtggtgccccgctatcctgtgaaaagaaaaaggctccaatntgtatatggtcctacgtcaatacactcccaaagata
ccggcaattctttgtgcgtgttgcggaacacatattagagntgtgaaaagaccaaatagtccacgccctttgtttcgtaaaagggccccctcccacgcga
gaggaaaatgggggttatcttatcgatgggaaatacgaccgagcccctctacgtaaagaaacaattaatttaaaaattcgtcaatatgaggataataatt
cnttgataaatagagggataaaatattggggtatcatcatattgtgtgaataaagtaattgttgtgattttgtggggctgacctacattcttcnctatag
aacgaggatgtatcctctgcatgttaaagcgttgcattccttcncttagttttcgccctttatttagttactttttcatattacccaacctntataatgg
ggggttaccccccaaatgtgtgacgtaaacgccggaagaagattttatgcggtttagcccaatgaataaaatggttgaaaataaaaactttacctcccct
3' EST: CF783042 [+]gttatagtcggggatctgctcaatgggccccactccggctacggcggggcacttgtcgtacaggtacttggagcagatctcactcactttcttagcactc
acagcgtcaatcctggcattcagttcctccaatgagatgcgctggccgagggtcaggacctggcgcccaatgtcctcgcacacgggggttgttccatcca
gctgggcgagcagggcagttttcatggcatttttagcttgtgccacctcgctgtcagttaccccagtacagaggcgcatccattctccctgggcgatgtg
caacatatcctcaatgttgtgtctgtctgtaacaaagtgcagtccgaacagccccgtgtcggactatcgaatgtgaaacggctggaaactctggcacagc
ttgtgttccgccgcccacgctggccactcggcttgacaggttcttgccgcctccgtacgtcaaatggtaagctatcaacgatttcattggctacaaaccc
ggggaatattatcctgaagccttggttccagaccgggccccttccacatcaatatcttaactggacccaaatgggaaggtccgccgatttcccagcatga
aatcccccgctgttcattgaaccctagcgcggtttgtctagctgctggcgataggttctcccttccccatttcccatcataacaaggccttaaaaaagtg
tgccgcttaatctaaagtccacaaaggtttcctttgtgaattcaacctcctccgggacccctatccatctattacaccccaagggggctatttaaagtgg
catcgcctcaattaaactccataacgaaatctacccccgtattttctcccctcccggggcgactattttcgtaccgtatccatacccctt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: