XB-CLONE-4354349
Clone Name: IMAGE:7026645
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Gene:
rps26
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723011 [+]
taattaatcccccccccccccgctcttttcagccctcgactccaaaatgaccaagaagcgaaggaataacggacgcgccaagaagggccgtggtcatgtc
cagcccatccgttgcacaaactgcgctcgctgcgtcccaaaggacaaggccatcaagaagtttgtcataaggaatattgtggaagctgcagctgtcaggg
atatctcagaggccagtgtctttgattcatatgcacttcccaagctgtatgtgaaactgcattactgcgtcagctgtgccatccacagcaaggtggtcag
aaaccgctcccgtgaagcccgtaaggaccgaacaccacctcccaggttcaggcctgcgggtgtaccccagagagctcctccaaaccaatgtaagacaacc
gtggctaagatgtatcttaccaaataaaacagtttaaaaacaaaaaaaaaaaaaaaaggatcccctagtttctaaaaacggcccgcccacccgcggttgg
aaactttggccgttggccccaacttgtttttattggcaacttttaaaaatgggttttacaaataaaaaaggcattttagccttccccacaaatttctccc
ccaaaaatataaaggccatttttttttttttcccccttgggccatttttataaagattttgggtgggtttttgtggtccccaaaaactccacctntcaat
aatggggatattctgtttataccctatggtctcatggggaaattcgggggacaaatgtaattttttcggagccgccaatcccccctcatggggctctctg
agaattataatcccccccacccgaataaaaaagagataaactcttggatttttagagcggaacacttgtcgttggttgaggtcggctacaaacaaaaaca
cccctatggtttgataaaatagatggtgagacacaatcttcgaggttgggcgggagaaacggacccccgaaggggtccctccccgcgg
3' EST: CF783047 [+]tttttttttttttttgtttttaaactgtttatttggtaagatacatcttagccacggttgtcttacattggttttggaggagctctctggggtacacccg
caggcctgaacctgggaggtggtgttcggtccttacgggcttcacgggagcggtttctgaccaccttgctgtggatggcacagctgacgcagtaatgcag
tttcacatacagcttgggaagtgcatatgaatcaaagacactggcctctgagatatccctgacagctgcagcttccacaatattccttatgacaaacttc
ttgatggccttgttttttgggacgcagcgagcgcattttgtgcaaaggatgggctggacatgaccacagcccttcttggcgcgtccgctattccttcact
tcttggtcattttggagtcaagagctgaaaccatctgcggggggggggaataattaacgcgaatgccacaaacatcagttacctgtcattttctctctgc
ccacaaatttggttgaatttaggaacttggcatcttattccttgggccaaaaaaagggaactctgctgtttaattttggtgtgaccccttaaatcgcagg
agcctcttatgggaccccgttttagacttaaaccgtgggccgcccttttcaaaaaaaattattcggggtgtctcctggagaataccccaaaaggccggaa
tttgcctctcaaattgggtccataaaggtctaatcccccccggaccaaaggaaaaacgtactcccggtttttttgaggggcttctttaacttacccccct
tttaattccccaacgaggaagttaaagtaacctttcggacgcttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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