XB-CLONE-4354341
Clone Name: IMAGE:7026642
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Gene:
jagn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783052
[+]
taattaatcccccccccccccccgggcggatgttgccgaagcgccgggttcattgagcgtgatcaataatggcctcccgtgctggccctcgggcaactgg
tacagatggaagtgactttcagcatcgagagagagtagccagccactatcagatgagtgcttctttaaagaatgaaatcaagaagctgatctacgcccac
ttggttatgtggctgctgatcgctgctcatatgtgagtgtctcatctaaaattggtctccaaggatttggtagccatgccataccaatggcagtacccat
acctcctgagccttgtcccgtccctcttcggtctcgtctctttcccccggaacaacttcagctatctggtgatctccatgataaacacgggactcttctc
cattgctcacctcatcatacgggacactaagagatgttctctatggcacaggcaactgataccgccaaggcagagatctaccgcattcatcgttaggttg
tctccgggcatggactgttatagttattttaattcatgggtgagtaggccagaaataaagtttcgaaggaatgggaaaaaaaaagagaataaaaaacaaa
aaaagacttgaagtgggaaaaacacttggattttgaaaaaaaaactacatttataggacgcaaattcaaagagaaaaaatatgtgtcttcgcggagaaca
ctcagaaagaaggggagaactttctgtcggtatacgagactcccctcatggggttgtacccaagggtggggtaaatatagcgagagaaggaggggagtgc
tctctttaaatgtttggtagtggtttaacaaaaataaagtttagggtttgcagtgtggtattatataattcantatgaaggtgtttntttaaatggcgtg
atatgatttgcgttttataacaaacggcgcgtgcggagcnacccgattgggaagnagctaggaagccctagcagtcgacgcagcagcacaccaggaaaac
cgaataaaagaaatagtgcgagcgataatagactaataatatctggccctttcatgatctccagagaggtgtgttatggggcttttatgttatttgtttt
ttcctaacgcccccggggggcgtagggggaggtgtttgcctcgcgaatgtgttcggggcgctgcgcgggtgatatagtcggtatatatgtggtgctccct
gcctagtgtttatgtttnttgaanaagggagatttataatttaataaaaaaatatatcanctaaaacccaaataagaaaggagctnaggaggggctgctc
c
3' EST: CF783051
[+]tttttttttttttttcgtattagttggttttattcttggctgaactggcgagaggtttacactatggagcacattaaagggcacatttacacattgatct
acagtttgtacacgtgtcgctgcatgcaaataccaagtacaaatgctcagaaaggaaacaggcctgggtacaatccagtagcaaaggcctatggtgtgta
aggctcatgcacaatgacactttgtaaggctgccaaactggttccatttagatggttccacaagtcaattacagaggggacatttaccctttaataaaaa
aacaagagaccacataaaagactagcagcagggtttatcgggcttctattcctccttaaacatacagtatttggtgagcacagaacatattgggggcata
tttggatttatgcagaattaggagctgccataatgcttgccagaattctataggaacaacattatgagcaatagattctgctcagttaaaccaagccata
gggcggctcccattaaattatagaaataattactcagatagcacaatttcttataaagcaatgattaaaaaaaaacaaaaaacctacccttcagttaact
gctcaggcgtctctgataggccaatacttggtcacgctcacctttgctcctaacaaggtattaaaatcctatagcagtgactatatagttcaaaattatc
tgtggggattcaccccatactgcaccttaaaggggtggttcaccattacttttaacgtttaatatattatagact
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: