XB-CLONE-4354339
Clone Name: IMAGE:7026641
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Gene:
calr
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723012
[+]
taattaatccccccccccccccgcttagtggttcctggacgctgtgcttgacaggtcttttgaaggggcatcaccatggcccgcgttgccttcctcatcc
tgccgctgcttgcggctctgtgcatcgcagatcctgctgtttacttaaaggaagagttcgctgatggagatgggtggtcgcagcgctgggtagaatccaa
gcacaaatctgactatggaaagttcaagctaagcgccgggaagttctacggagacgcagagaaggacaaatgtctccagacaagccaggatgcccgcttc
tacgccctgtctacccgctttgattccttctccaaacaagataagacccttgtcatccagttttccgtaaagcacgagccaaaactaaactgcggcggcg
ggatacgtgaagctttttccctcggaaactggaacaatcccgaaatgcaccggccaaatcaaagttacacatttcttgtttggggcccttaacattttgg
cgggcccccccctaaaaaaaaaaagggtgccaagtttttattcttctccctatacaaaaaaacaaaaaaaaaacccttttaaaaattaaacaaaagggaa
tttacccccgccgccatggggaataaaattattttttaccccctatttttgaatcagcccttttaaattttggtggcctccccaaaaaaatacccccctt
atataaaatttaaaaaaatttgcgaaccacactcacaaggggttgggaaaactccgggaagaccccctttaataaaaaaaaatttgtgggatattttctt
gtgactacccccgaaaaaaaaatataagggagccctctgggggggcgcgcaaaaaatccccacttgactcttgggc
3' EST: CF783053
[+]ctttcctttgtaatctgggttatcgatctgacggggtttccattctcccttgtagtctgggttctgtataactgggggctcccactcgccatccatctcc
tcatcccagtcttcaggcttcacggcatcagggtctggaatgtgttcagccttatcccaatcctctggcttcttgtcttctgggtcatcgatcttggggc
gctcgtcccagtcatcgggcttcttggcctctgggtccttaatcttcttggctggcaggaagtcccagtcctcctccaggcttccggattccaccttgct
gttgtcaatctttacttcataggtgttgtctgggcgcacgatcaaggtgtacaagtgggtgaaagaatcgtccctgcagcggatatccttgttgatccga
aggttcttcttcttgtactggaagataacatcgcaccttctttgtgggggggccgcaaaatgtcaaggtcgaaacatgatgtagtaatccgattcaccca
ggatctacgaactgctccacctcttctgggaatagccttccgatattcccccgttcacagcctttgtttcccccttttgtctataccttataaacttctc
acaaactgtttttttactctttttcccccatttttcaacttaacctcgttcataactaaggctttaaaataccaggtattctatggatttgccatttgtt
cgccaccttcctttataccccgcagtcctagggcaatatacatcgacactttccccatactatatacaccttttctacatatccggacaccctctctcaa
catacttactatttaacatgcacgccgcttttacctcaacctttttattcgcctcacctttcccaactcttttttttatatac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: