XB-CLONE-4354336
Clone Name: IMAGE:7026640
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Gene:
vti1b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783055 [+]
taattaatcccccccccccccgaagtatcagatatgtcatctgagcaatatgagaaactccatgagctctacaaagagctatatactaagctacaaaaaa
tccaggagagactgcagagctgctatggagaggaaaagaaaaaactgcttagagaatttagtgaaaagcagaaggaagcaaatgaaatgcagcaggtcaa
ttaagagcttttttttgagatctggttaaacttcagatggaagcaaattataatgcgcttgaagttagcaccaaagaaagagaaacctacagtgtggaaa
atgagcatagtacacgcttagaatcccaaagagcacttcttctccaaggcactgaaagcttaaacggagcaacggacagtattgctagatctcatcgtat
agcagctgaaactgatgccattggccctgatattatagaggagctaggcgaacagagagaaaaattggagaggaccaaaggacggttggtggggcacaag
tgagaatctttagtgaaaagccgaaaaatcgctgcgggtccctggtcacgcgaagatttgtgacaaaacaaaggtttcgggcggggcgaatcataaagta
tatttctgggaaaataaaggggaatcccattgggtggggctgtaaatccctaaattaattcaagttctcttgtcagatcaaaaaccgacgttggaaaatt
tttttggggagtctataaaaaacatagcacttcaaatctgaggtgatgtgttataaagttaagaggagaggtgagattcgtggggggaaccaagaaaggg
tgttttgtgttaatatggggaggacccggttttagtagggaggggggaacagagaaggaagacctaggcaactatgggaataccaatttgacgaaattcg
caaataaagagctggggacactaacccccataaaatttattgggtggcatcaggattttttgaggaacttatgtatgttttattttggaaatcatgtctc
caggaaaagatataatatttgcgagaataagggggggagagaaaaggtagggtctgtgtgtccgcgcgggggagtggtggggggtaccacgcggtaggaa
tgtttggtttg
3' EST: CF783054 [+]tttttttttttttttccatctcattgatcaaactgcaccctttactttcattattattaaatacaaacattattttaaagtctattaaaacaaaaccttt
tgagacaagcaaaaattgagtataatcattcagttttatatacagtataagtaaatccaaggtaaactggatcacatcatatatcagtacactcctttag
aagttttcttgccatttgcaaacaatcactgtggtcatgatccagaaaaaaacaattcatcagtacagaataatatatgagaagttaaaaaaaaaagtac
aaaagtgaccaataatatggcttagcagctttttgattcaatggctcaaacgcagcttcctcttcactttataacagcgccattttacaaaaaaattttt
ggggggccccaaaagaaaaaacccttttttcccaaaaaanncaccacgnaaagacgccccctgggttttattataaacaaaaaaaaaaaaaatcnccccc
cggngggggggggggggggggggggnaaaaaaaaaaattttttttnntttagagaggagaaaaaaaaagagaggcgcccaccccccccaccccggagggg
gaggaggggggagtcttttntcttttttcttttgtnncggnaaagaaaaaaaaaaaaaaaaaatntttagttattttgtgatatgaggagagggncgcgg
aaagccaaggaaagaggccccn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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