XB-CLONE-4354326
Clone Name: IMAGE:7026635
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Gene:
slbp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723015 [+]
taattaatccccccccccccccgagacctacgagaacatcttgaaggcaggaggtcaccggctcagggatcgcagtcatgtcgcaccactggcgcactaa
caccgaggaacccaggcctcatgcaccatctcgctggtcacaaggaaggaagcgacgctcagacggaaaactgagacgttacgatgatacagaatccacg
gtatttgatgataagccttccgaggaacctagcgagaggcctgacagctttacaacccctgaaagccataagccagtggcaagatgcaaagactggggca
gtgcagtcgaagaagaggagcagatgagggaaaaagttggtcaagacatagcacgatacaagaggaaacttctaattcacgaatttggtggaagagaaag
aagatcatcatctggaagttctgattcaaaggattcatctacacatggagagatggaaactgacccagctgttcttacacgcagacagaagcaaataagc
tatggaaaaaatactattgcctatgacagatatattaaggcagtgccaagacacctccgagagcctaatgtgcatcctagaactcccaataagtttaaga
aatacagtcgcagatcatgggaccagcaaatcaggctgtggagaattgctctacatcaggggggaaccttcctgcagaaagaaacagtgactttgcagcc
tttttacggaaaaaaaacatgattctggtgtggaaaaccaagctcacaagaaacagccttattcacagggcacctccaacgaaaagttcccgggaaaagt
ttaaagccaaaaaggccttgaatttataaatcttgggaaacccctgcccggtatactggaaaggaaagaacattttgtgtttagatttaaaccataaaag
gtggt
3' EST: CF783061 [+]tttttttttttttttgcagaaaacatgttaacccaattataggcatttacatttattaaacctgttatgcaagccaccatctgtttggtctgacagtggc
caagtgaagcagtcattactgtaggtggcgggactgcggatacagctcagctgctcccatatcaagtgcttgcgaagaacgccacctagcagcctactac
cagcaccaaaatatgtgcattggttaaggcagctcagatatattgtgaaatcttactgtatagatgcgcaagagtggacctgggagtgaaatctaccagt
ctctgctgtgtgtgtaaacagggttcatgccaaatagcaatcctgagccctttcttctggaaaagcactgtatgttaactaacaaagtcttcctcagtac
cgcagggttccagatctaattcagcctctgcttcaactttccggaccttcgttggagcgcccgtgaatatgctgtcttgtgagcttgtttccacacagat
catgtctttttccgtaaaacgctgcaagtcattgccttcttccgcaggagggtttctctgcatgttcaagctttttcttttcagccttttttttgttggt
ccctaagatcctcgtccctgttatttccctacactcttcgggggagattccaagggaatccacatttgggtttctatggaagtggtattttggccactgc
ctataataaatttcttgcccccagccttttccgttctttcttccataaatcttgatctccctcctctgtttttcgcttataaaaacccccccggtgccaa
ancttcctttcctctccaaaagtttgtaaaacgaaaccctcattgaatccccn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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