XB-CLONE-4354322
Clone Name: IMAGE:7026633
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Gene:
chchd2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723016
[+]
taattaatcccccccccccccagcagagagaggactgtttgaaggacacggaacgcggaatattgggctaaacaatctttagccatgccgagaggaagcc
ggagccgcacatctagggtagcccccccagccagtagagcaccagcaatgagaccagcaccccctcctgctgctcacccacctgcccctgttgctcaggc
tccttctgcccttggtccagcagcagcagcacccagacagccaggtctcatggcccaaatggctacaactgctgcaggggtggctgtgggctctgcagtg
ggacacacaataggccatgcaattactggtggttttggtggaggaagcagtgctgagccagcaagggcagatattacataccaggaaccagcacagccta
tgtatcagcaacagcagcaatctcagtatacaccttgtcagtatgagatgaagcaatttctcgaatgtgcgcagaatcaaagtgacctaaaactctgtga
gggatttggagaagttcttaaacaatgccgctttgcaaatggcttataacaaacctggacatgaagaagaaatgtatgtagatgagcatggtgcggagga
attgacctgtgatgtccagtttattcagttaattgcatgaggctttattctgatggcacataggcatctttccccacagcatatctcagcccaacaaaaa
cattaatggaaactgccacctgttgccctcccctattttcgtaaaatgggaaaatggccccgacacgaagcccttttaaaggggggttaatttttatacc
aatttttctcgagtgttggtggaaattagaaaattttttgtttttttttctaaaaaaatgagaaataatacacggtggcccaattatttgagtattgtgg
gta
3' EST: CF783063
[+]tttttttttttttttaacatggaaatagtttatttcaaaataagaaatgctataatcaacagcaacaaacaactcactggccaaaatgcagtcgtcatag
ccacaatcacctagtgcattttttatttccagatgttccccaccagctgttatttttctggtcaaaatagttgagtggattagatttaagacaaggttaa
ggctgatagcatattgacaattttctcgaccaacataattgagctaaaggtggagaattgccctaaaatgttcctgctgtctcagtcattgaatgtgcac
attgacaacctgaagatgttcactgtagcttctgattgaaagcaccataaagagggcgatttattaacgttagattttgatttttgttcacaaattgggt
tttttttttagagctaaaactgcacaattcgagttgaggttcaaaaaccaaaaactggtatttattaagtgcaataaacccaaaaactcgaaaaaaaact
tatatagaaacttgcaagctttaaatggcaaagtcaatgggagtagtcctaggccaagtcaagccatattttcaaactctaatttttcaagttctttaag
tttgcaagctccaaatattcaagtttttttatttgaacgagttttccttattaataaataaccaaccaatcaatatgcacgttttatttcgatttaaaaa
aacaaaattttattttccaaaactcgaaaaattaataaataacccccttttaaagggttctgtccgggccatttttccccctacgttaaaaagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: