XB-CLONE-4354320
Clone Name: IMAGE:7026631
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Gene:
rpl19
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723017 [+]
taattaatcccccccccccccgctttccgagctgaagcagagcggccgccatgagcatgctcagacttcagaagaggctcgcctccagcgtgctgcgctg
tggcaagaaaaaagtatggctggatccaaatgaaaccaatgagattgccaatgccaactctcggcagcagatcaggaaactggttaaggatggcttgatc
atccgcaagcctgtgactgtccactcccgtgcccggtgcaggaagaacacccttgcccgtaggaagggaagacatatgggcattggtaagagaaagggta
ctgccaatgcacgtatgccagagaagcttgcttggatgaggagaatgaggatcttgcgccgcttgcttcgccgttaccgtgaatctaagaagattgacag
gcacatgtaccacagcctgtatctgaaagtaaagggaaacgtgttcaagaataagcgtatcctgatggagcacatccacaaactaaaggctgacaaagct
cgcaagaagcttttggctgatcaggctgaggctcgcaggtctaagacaaaggaggcaaggaagcgccgcgaggagagactgcaagccaaaaaggaagaaa
tcattaaaacactttctaaggaagaggagtctggaaagaaataaatcccacggcccctgcatttgtgtatatagagtgctgctttgtgcaaactcaataa
aaaactcacccacaccgtgaaaaaaaaaaaaaaaag
3' EST: CF783064 [+]tttttttttttttttcacggtgttggtgagtttttattgagtttgcacaaagcagcactctatatacacaaatggcagggccgtgggatttatttctttc
cagactcctcttccttagaaagtgttttaatgatttcttcctttttggcttgcagtctctcctcgcggcgcttccttgcctcctttgtcttagacctgcg
agcctcagcctgatcagccaaaagcttcttgcgagctttgtcagcctttagtttgtggatgtgctccatcaggatacgcttattcttgaacacgtttccc
tttactttcagatacaggctgtggtacatgtgcctgtcaatcttcttagattcacggtaacggcgaagcaagcggcgcaagatcctcattctcctcatcc
aagcaagcttctctggcatacgtgcattggcagtaccctttctcttaccaatgcccatatgtcttcccttcctacgggcaagggtgttcttcctgcaccg
ggcacgggagtggacagtcacaggcttgcggatgatcaagccatccttacccattttcctgatctgctgccgagagttgggcattggcaatctcattggt
ttcattttggatccagccatactttttttctgccccagccgcatcacgcttggaggcgaagcctcttcctgaagttctgagcatgcctcatgggcgggcc
gcctctttcttcacctcggaaaaccggggggggggggggcatttaattaacttcgtgggccaaaacaaattaggaagccttgtatttccggtcccgggtt
taaaccttagggtgcactttggac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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