XB-CLONE-4354306
Clone Name: IMAGE:7026624
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Gene:
vwa5a.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783072
[+]
taattaatccccccccccccccgtctactgccccagctcctccatctactgccccagctccctgcactgtgtgctggcccttctcctgtaattgagacaa
catggattcctgctgtggccttatcatctcctgctccaaggtccccgtgccgctccgtgggatctcggtggaggtgcaggtaaaaggcttcgtggctgat
gtatgtgccacactgaggtacaagaacacggaggagaagcccatagaagccatctttgtgttccctctggatcaagactctgccgtgtacagctttgagg
ccacggtggaagggaagacgatagtggcagaactactggagagggagcaggcccacaaaaccttatgaagaggccattaaccaaggccaggaaccttttc
tgctggaggaggataagagctctggtgacattttcagctgcggcgaatggaaaccttccccctgggcaaagatgttgaaggtgaaaactaaaacttacat
gacgggaaaccgcccatattgaaaatccaaaaaaggaaggttgtggcgtctatttgtgcctttcctaacatggtccctggggaccccctaaaatagcacc
cgcaaaaaagaaatcaccggagatatggtggcggtttatacagggcttttctcggaactgccagcggaatttgcgtccgcgccattgaaaatagaaaatt
ctctgctgtgagataaagcgcggtttggccacttcgtagaaaaagagaccgcgctgaaataattttttttgtgaaagagacctccgcgggttcacctaaa
gtagaggggcgcgaaaaaaccctctctcgcataatatgacataanttgtggtcttcgttttcccattaatctccgcggggttgtcttgcgttctttttcc
ttgtgggtccctcctcgatctttttactggaattaacacattcctctgtgcaatgtgtggggggggcnagtacaatacttgtggcgtccagagaaaaaca
taaaagaagtgagatggtctcccccccagggagcctctccaangagagagagaaaaacaaaaaaattttctgtggtgatataaaaaanacactatctttg
ggtaaaaataaaatgattnttgagtgtcttaaaatcatatatctgggtgaggagagagagaggagaggcacgcacccacgaaagagctccccctgagttt
gataaaattagaggaggagactctttacctagaaaaaccccggcgtggaaagaatgagtatattgacatacagggtggcacaacccccaattggaggcct
aatttctctt
3' EST: CF783071
[+]ggattttctctttcagagcctcttcggacatttcaaagacggaagccaattccgagttgaggttccaggacccgtctgcgttctgcagggagatgagctg
gaacagagcccgttctgctttggcctcggcgggagcagatgtggcgccgtcgctcacagaagcggggtctggccaataacaaacttctacttttggttca
ggcgacccctcggcgggagcagatgtggcgccgtcactcacagaagcggggtctggccaataacaaacttctacttttggttcaggcgacccctcggcgg
gagcagatgtggcgccgtcgctcacagtagcggagtctgaccaataacaaaccgctatttctggttcagacaacccctctgccggaccagatgtggggtc
agcgagctcaggagtgggcaagagtggcatggctggcataggagtgggtgcagggaaatgacaaacagcaatttggggtttatacccccctggtgccgcc
atactgcaagcaagcgctcttggtggcccacctggcatcattcttggcattggcatacagcaagccattgccactggtgcaccatagagtgggatggacc
tctggaccggggggcctttcactgggttcttggtttccttattgacagctacatangccgtgagggggggagatgaccccggaatggacactggtctcca
caatctgtttctgcactttctccgatttctccttctaggttttcccccaccaatcaggtttagagatgaccacctttgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: