XB-CLONE-4354300
Clone Name: IMAGE:7026621
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Gene:
mybph
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783076 [+]
taattaatcccccccccccccggttgcaattgctgagagcagagtcagaaccatgccagcaaaggctccaattaaaaaatcccctgcagctaaggctgct
ccagctaagccacaggcagctcctgtacctgtacctgagccagtagaggaagtccctactcctgtgccagaagcccctgctcctattcctgagcctgttg
agaataccgttcaagaggcacctgcaccacccacagaacccccagcaccagaggcagaagtttctgtcccagcaccagaaacacctacaccagctgcaga
accagaaaaggctcctgaagtagttgaaccacccccacctgaaccaaaagaagaaccaccaagtgagcctacagatttaacagtggatgaaattgatgac
acttcaatatcccttaaatggaaagctccagagaaggaaggaactactgccctggatggatacttactggaataccaaaaagaaggcagtcaagaatgga
ttgctgtgaacaaagagccaagtgtttcaactcgatacaaaattcagaatttgaatactggtgataagataactgtttgtgttaaagcagttggcaaagc
aggaagcagtgctccaagagcacttgaaaaatttgttcaaattcgagaaattgtggatcgtcccaaaatccgtcttccaaggtatttgcgtcaaacattt
tgtgaaacaatgtaggagaagcagtggaatctagtgatccccgttcccgggttaagcccaagacccccaggttaaactgggactaaaaaatgggacagcc
ttcctgggaccttaagcattgcaaacctgtccggaaaacagtggagaagtggaaaacttatttcttttttatttccgaaaaggcgtgaaagcggtaaaag
aattctgggggaaccaatatagaaattgtgacttggtat
3' EST: CF783075 [+]tttttttttttttttgtgctgaataaattctttattataagtcatatgaaatacaataaatacaggacatcatttgtgttgagtaaactgtatgttattt
tgtgccttcattaatttcattcagcaatcttctatttgttatttatgtcctgtttactgtggcactctaacatccagcctgcaatctacacttgcttccc
ctaatgagttgattgctttacaggtgtaaactccaccatcaaaaggacagggcttgcgaatttcaagagagcagactccctgattaacaaggcaccgaaa
tttagggtcctctctaatttccatttgattctttagccacacaattctaggctttgggcttccacggacacaacagaacagtttagtgctatatccagtt
gttgttgctctattagcaagcggctgggtaaattttggtggctctgcaaaatctcgttccttgtactctgggggcttataagtgattcctgttttcttaa
taacagcagcatctttaattactgcagccttttcacttagtccacacaagttttcagaaaatactctgaagtaataagaatttcccatgatcaggtctga
aacagtgcagttagtctggcgataatgctcctgtacagtaaccattccccagttttcttgtctgctttttgcacagtgtatcctgtaatatctgaatttc
catcatctaatgggggagtccactcccaagctgcattaaatctcccaaacatccagaaagcttaatactttttttggtgggcccagggccgcttccaaca
aattttttatcctcaatcct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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