XB-CLONE-4354295
Clone Name: IMAGE:7026619
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Gene:
gpat3
,
chchd2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080141
[+]
CGAGCAGAGAGAGGACTGTTTGAAGGACACGGAACGCGGAATATTGGGTTAAACAATCTTTAGCCATGCCGAGAGGAAGCCGCAGCCGCACATCTAGGGT
AGCCCCCCCAGCCAGTAGAGCACCAGCAATGAGACCAGCACCCCCTCCTGCTGCTCACCCACCTGCCCCTGTTGCTCAGGCTCCTTCTGCCCTTGGTCCA
GCAGCAGCAGCACCCAGACAGCCAGGTCTCATGGCCCAAATGGCTACAACTGCTGCAGGGGTGGCTGTGGGCTCTGCAGTGGGACACACAATAGGCCATG
CAATTACTGGTGGTTTTGGTGGAGGAAGCAGTGCTGAGCCAGCAAGGGCAGATATTACATACCAGGAACCAGCACAGCCTATGTATCAGCAACAGCAGCA
ATCTCAGTATACACCTTGTCAGTATGAGATGAAGCAATTTCTCGAATGTGCGCAGAATCAAAGTGACCTAAAACTCTGTGAGGGATTTGGAGAAGTTCTT
AAACAATGCCGCTTTGCAAATGGCTTATAACAAACCTGGACATGAAGAAGAAATGTATGTAGATGAGCATGGTGCGGAGGAATTGACCTGTGATGTCCAG
TTAATTCAGTTAATTGCATGAGGCTTTAGTCTGATGGCACATAGGCATCTTCCCCAACAGCATATCTCAGCCAACATAAACATTATTGAAACTGCACCTG
TTGCCTCCTATTTACGTAGATGGAAAATGCCCGACAGAGCCTTTAAAGGGGTTATTTATCAATTTTCGAGTTTGTGAATTGAATTTTGTTTTTCTAAATC
GAATAAACGTGCATATTGATTGGTTGCTTATTTATTAATAAGGAAAACTCGTTCAAATAAAAAAACTTGAATACTTTGAGCTTGCAAACTTAAAGAACTT
GAAAAATTAGAGTTTGAAAATATGGCTTGACTTGGCCTAGGACTACTCCCATTGACTTTGCCATTTAAAGCTTGCAAGTTTCTATATAAGTTTTTTTTCG
AGTTTTTGGGTTTATTGCACTTAATAAATACCAGTTTTTGGTTTTTGAACCTCAACTCGAATTGTGCAGTTTTAGCTCTAAAAAAAACCCAATTTGTGAA
CAAAAATCAAAATCTAACGTTAATAAATCGCCCTCTTTATGGTGCTTTCAATCAGAAGCTACAGTGAACATCTTCAGGTTGTCAATGTGCACATTCAATG
ACTGAGACAGCAGGAACATTTTAGGGCAATTCTCCACCTTTAGCTCAATTATGTTGGTCGAGAAAATTGTCAATATGCTATCAGCCTTAACCTTGTCTTA
AATCTAATCCACTCAACTATTTTGACCAGAAAAATAACAGCTGGTGGGGAACATCTGGAAATAAAAAATGCACTAGGTGATTGTGGCTATGACGACTGCA
TTTTGGCCAGTGAGTTGTTTGTTGCTGTTGATTATAGCATTTCTTATTTTGAAATAAACTATTTCCATGTTAAAAAAAAAAAAAAAA
5' EST: CF783079
[+]taattaatccccccccccccccgagcagagagaggactgtttgaaggacacggaacgcggaatattgggttaaacaatctttagccatgccgagaggaag
ccgcagccgcacatctagggtagcccccccagccagtagagcaccagcaatgagaccagcaccccctcctgctgctcacccacctgcccctgttgctcag
gctccttctgcccttggtccagcagcagcagcacccagacagccaggtctcatggcccagatggctacaactgctgcaggggtggctgtgggctctgcag
tgggacacacaataggccatgcaattactggtggttttggtggaggaagcagtgctgagccagcaagggcagatattacataccaggaaccagcacagcc
tatgtatcagcaacagcagcaatctcagtatacaccttgtcagtatgagatgaagcaatttctcgaatgtgcgcagaatcaaagtgacctaaaactctgt
gagggatttggagaagttcttaaacaatgccgcttttgcaatggcttataacaaacctggacatgaagaaaaaatgtatgttaatgagcatggtgcggga
ggattgacctgtgatgtccagttaaattcctttaattgcctggaggctttagtcggatggcacataaggcatctttccccacagcatatctcagcccaac
tttaacttttttgaaactgcacccggttgcctcctattttacgttaaaagggaaaatggccccaaaaaaccctttaaagggggttattttatcaattttt
cgaagttgggggaaattgattttttgtttttgccacatccgaaaaaacgggggcattttgcattgggttcgcctttttttttttataaagaggaaaaaat
cgc
3' EST: CF783078
[+]tttttttttttttttaacatggaaatagtttatttcaaaataagaaatgctataatcaacagcaacaaacaactcactggccaaaatgcagtcgtcatag
ccacaatcacctagtgcattttttatttccagatgttccccaccagctgttatttttctggtcaaaatagttgagtggattagatttaagacaaggttaa
ggctgatagcatattgacaattttctcgaccaacataattgagctaaaggtggagaattgccctaaaatgttcctgctgtctcagtcattgaatgtgcac
attgacaacctgaagatgttcactgtagcttctgattgaaagcaccataaagagggcgatttattaacgttagattttgatttttgttcacaaattgggt
tttttttagagctaaaactgcacaattcgagttgaggttcaaaaaccaaaaactggtatttattaagtgcaataaacccaaaaactcgaaaaaaaactta
tatagaaacttgcaagctttaaatggcaaagtcaatgggagtagtcctaggccaagtcaagccatattttcaaactctaatttttcaagttctttaagtt
tgcaagctcaaagtattcaagtttttttatttgaacgagttttccttattaataaataagcaaccaatcaatatgcacgtttattcgatttagaaaaaca
aaattcaattcacaaactcgaaaattgataaataaccccctttaaaaggctctgtctgggcatttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: