XB-CLONE-4354292
Clone Name: IMAGE:7026618
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Gene:
uqcrc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783081
[+]
taattaatcccccccccccccgtcttcctgacagcggctgaaggagagagtgaggccatgaaacttgctacgggtgcccggttcctctctaggaggcttt
actctgcaaaagctgctccaaaacccgaagcaagtggacaggcacgccttaacccggaggagctacagctcacaaagctaccaaatggattggtgattgc
atctttagaaaattattccccatcatccaagattggcgtatttataagggcaggaagcagatatgaaaatgccagtaaccttggggttaaccatgtgctt
cgtcttgcatccagcttgacaaccaagggtgcatcagcatttaaaataacccgtggaattgaagctgtcggtggtggattaagtgtgacctctacaaggg
aaaatattgtttattcagttgaatgtctgcgagattatgtagacactgtaatggaatacctcatcaatgttaccactgcaccagaattcaggagatggga
ggtatctgatcttcaggcaaaagtaaaacttgataaggcctttgcatatcaaaacccacaagtcggagttcttgnaaaccttcacgtagcagcatacaga
aatgcacttgctaacgccttgtactgtcctgactacagacttggaaaaagtacatcagatgagttgcagcagtttgtacagaaccattttacaagcccaa
agatggcactggttggaataggtgtgagccactcggttccttaacaagtggggggagcagttcctgaatataaagaatg
3' EST: CF783080
[+]ctccttatattcaggaactgctcccccacttgtttaagaaccgagtggctcacacctaatccaaccagtgccatccttgggcttgtaaaatggttctgta
caaactgctgcaactcatctgatgtaacttttccaagtctgtagtcaggacagtacaaggcgttagcaagtgcatttctgtatgctgctacgtgaaggtt
ttcaagaactccgacttgtgggttttgatatgcaaaggccttatcaagttttacttttgcctgaagatcagatacctcccatctcctgaattctggtgca
gtggtaacattgatgaggtattccattacagtgtctacataatctcgcagacattcaactgaataaacaatattttcccttgtagaggtcacacttaatc
caccaccgacagcttcaattccacgggttattttaaatgctgatgcacccttggttgtcaagctggatgcaagacgaagcacatggttaaccccaaggtt
actggcattttcatatctgcttcctgcccttataaatacgccaatcttggatgatggggaataattttctaaagatgcaatcaccaatccctttggtagc
tttgtgagctgtagctcctccgggttaaggcgtgcctgtccacttgctttcgggttttggagcagcttttgcaaagtaaacctccctaaaaggaacccgg
gcacccgtaacaaattttctgggcctcactctctcctttcaacccgctgtcaagaaaaacgggggggggggggggaataattaaactccaggtggaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: