XB-CLONE-4354276
Clone Name: IMAGE:7026611
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Gene:
depdc7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783091
[+]
taattaatcccccccccccccccccgagtgcagcagtaggagagcggaatggccacagtgcgggagaaagcggtgtctttgaatctatgtgcgacttaca
gccccgcacagcgacccccaggtccaaatggaagccatagaccattccgagccacctatatctggagcagcatcatccaagctctcacacaggtagaagt
gaagaagaggcggcaccacctgaagtaccaccatgactgctttatcagctctgaggccgtggacgttgtgtttgcccacctggtgcagcacaagtacttt
ggggatacggagattccccgctccaaggttgtgcgagtgtgccaggctctcatggactgcaaagtgtttgaacccatcgtgacggcctgcatgtttggga
gggagaagaagaggacggtgttcgaggatagtagctgcagcctgtaccgatttataaacaattcaagccatcttggtgcccaggtggagaaaagcaatgg
acgttgtacccctcagagacccaaacactcaagcttccaatctgcgccacttaagtctccaagcctggaagatctgtgggataacctcagtctgacagct
gcagacccaacgcacataaacctcacagctaacctacctcccaaagttgtcggtgaagtttggcaagaaaagaccatccgccgacttctggcagcttgta
aacctacctctgcctcgattccttgttgggagtacactccctggttgctccccgaaattcccccaagttgaaggggaagaagaaccttaaaccctctacc
cagtaaactaacctggggaccggggggaaaatccctttaaagcccctttccagggggaagggcctccgggggcacaaaaagtaagttggggggtcttcca
aaccacccgcgggggggggggaaattggatctttggggat
3' EST: CF783090
[+]tttttttttttttttaggaaatatttgaaagtttattaaatgtgcaaaaacaattagttattcaatatattaacaaattcatatttacaaagacattgta
cagatctatacacagaggggctgaggtcgctacgtgtagacgctgctcacgcggtctccgaagtactggataaaagtctctgggtggctcttgtagaact
ggcccaggagccgtttcttctctttgggggagaggctggtgttctcgtaaatggtcttcagcaaagcgcagagttccgtccgagtgttgttctgcgtggc
tgaatcaaattcccgtttgtccaccctttggcagaaagtataacctgtgtctcggtctggatcgctgccttgctgaattgctaccagtttatcgctgacc
atcttgtgcaaggagccgggtatcttaaacacatccttgtgatggtccagtatgaataataccagcaggtcgcacttccccttggacaggttcttattgc
agacgacggctctagagaacattcttttcacagtcatcctgttgtcagtctcttcgtgcagtcggaactctgaaggatctgccgcaagagccataaaata
caggaggcgtctgaactcctcgcggctcggggagtccaacagcttcaggcagagctgggtcgcctccagagcgggttcattctttccattcacaagcagc
tcggcgatgccagtatgaatatcgaacagctggtttctgagcagcgggggaccggttctgggtagtgttttgccgatagtaatcaaacaggagcagcctt
ccattttan
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: