XB-CLONE-4354273
Clone Name: IMAGE:7026610
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Gene:
hnrnpab
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783093
[+]
taattaatcccccccccccccggggagagtccatcttggaggaatttggcgattgttggcgcttgtcctttcagtgcatagaaacccctcacaatgtccg
ataccgagcagcaacagtacatggaaactggcaccgagaacgggcacgaagcgtgcgatgctgaggccgcggagagtaaggtgcccgggggccagaacgg
cgcggaaggcgatcagatcaatgccagcaaaggcgaggaggacgcagggtgcgtacctgatatttcttcccctttaactgaaggagtgaaaatgtttgtt
ggtggcttgagctgggatacaagcaaaaaggatttgaaagattacttctcaaagtttggtgaggtgtctgactgcacaatcaagatggacccaaacacag
ggagatctagaggttttggatttattctgtttaaggatgctgcgagtgtggacaaggtattggagcaaaaagaacacagattagatggcagattaattga
tccaaagaaggcaatggcaatgaaaaaggatccaataaagaaaatatttgttggagggctcaacccagaagcaggagaagacaaaataagagaatacttt
gaaacttttggagagattgaggcaattgagcttccaatggaccccaaaacaaacaagaggagaggctttgtgttcctcacatttaaggaagaggaacccg
tgaaagaagattttggangaaaaaaattcacaacgtcagtggaaagcaagtgtggaaatcaagaattgcccaccaaaaagaagtgtaccacgcaacacgt
atggggggcagaaggtgggcaccttttgttggaagaaggtgggccaaaaggggttaaaaactccaggggcccaaaaattgggaa
3' EST: CF783092
[+]tttttttttttttttcttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttcccc
aaaggggggttttttttaaaaagggggaaaacccccaaaccccgggggggggggaacccccccgggaaaaaacccctaaaaaaatttaattcccaaaggg
gcccaaaaagaccaaaaaaattccccccccccacgggggaggggggggacaaaccaataaaaaaaaaaaggggggaaggggccccgggccccccaagaaa
aaccccccggccaacgggggaaaacccccttttttcccccaaaagggccccccccaagaaaaattggccctttaaaaaaaacccccaaaggggggggttt
tttcccctgggaaaaaaaacccccctttaaggggccccgggggggggggaaacccccgagggcccccccccctggggaattttcccaaatttcccccccc
cccaaaagaaaaaaaaccggggcccatttccgaaaaccccctccccaaaaaagttaattttcccaaaccccccaaaaccttggggggcaaaaccccccac
acccggtggtccccaaccccgagaacccaaaaagtggggaacccccaaaatccaatggggggggagagttttaacccccggggaccttttttaaaaaaaa
aggggcccccctgggacaaaaaggggggggggggaacccctccttgggggggggggaaacttgttatccccccctggttccccacgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: