XB-CLONE-4354270
Clone Name: IMAGE:7026609
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783095 [+]
taattaatcccccccccccccagttgttgttgaagagtggaaggggaaggagcggagacttcgagcagcagaaaatcctcaacattcgcagttatttttt
gttgtttcagcgttccgtcttactcaaaaccatcgctttcttctgtcagctacttttacgcgttttgcgaacctgcccctatatgaaggtatttataact
agctacaatactgtgctattgtgcctccctgtttcttagttatatatgtatttctttgtatatattagtagagaacgcagcggctgcctttagttttctc
agtgggttgggattctgtttaatgagcacagtgaaagtatcggtatcagccagtttaaaactaagcgagttcgctagaaaaccaagtgggagaaactata
gctattatatatttaatcgcttatggtacgtgatattgtgcgacttaaacgcgctgttagtgtgggtttatttgccacgtctcgccgggcttagcgatag
tctacaaaatggcgcctgctggttcttaggtgggcgtggcttacgctgccggtcgccattttaaactctatgaaattccttccaaattcaagatggctgc
ccaaccttttatgggcggggctagaatgttctcgtaaatctgtatgacgatacctgtggttatttctaagattaaccacaatgtaatatgagcgtctagt
acaatgtaggcgtattccatttgtccggaattttttttcagcccccttttcttttcaaatcattttataccggaattaaccttaatgattcatgggccta
tttttaaaaaaaccccggatttttcaaatgggggccttttaatttcccccttggctggtgggggagaaaaggggtttccttaaaaggggcagaaattttt
cc
3' EST: CF783094 [+]tttttttttttttttgttttttttttcaatctgtaaatttaattttgaaaattgttacacataaaatgataatctgatacaatcaattcttacttgtaaa
tacatttaagactttgcagatgtcacaagtcactcaaaaccattactatctgaaaaaaaaaactacctacggaaacactgggaacagctgaacgcattaa
tattacagatttcattaagtttctactcaagcaagaatgcaacagcataattgaagaaactgcatgacaaaacttgaagtaattactagtaaacttgttt
acagggtccctatactgcttataatgagtaatgtggaactatactactaacaattgttagcattattgtagaatgtgttattttaaagaaagtcttgcca
caactctccaagagactacaatacactgcttagtggcaagaattcaatcatttctattagtggtgcaaatcctacagaagagggagtaggtaaaacggtt
atgaacaggctggtttagtttaattcaaatgtgggcttaattgggccaaaaaatgtaatgtaaggggtggggttattgcttaccacgggactgacagtac
actgccatcacagggaccaagctgcattgaagatgaggtatcaaaagaagctatccaatgacaactgaacaaaatggaagcaaacttggttaacatctga
aaaataatcccatttaatgngaaaaaaggggtgaaactgtggactaaaataaaaatcanggggaatgacatgnttaaaatatttgctatagtaacacana
agctgattaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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