XB-CLONE-4354267
Clone Name: IMAGE:7026608
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Gene:
rpl3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783097 [+]
taattaatcccccccccccgctctttcagttcggtcagggcttgagatgtctcaccgaaagttttcagccccccgacatggttccctgggcttcttgccc
cgcaagcgttccaagagacatcgtggcaaagtgaagagcttccctaaggatgaccagagcaagcccattcacctgactgccttcctgggctacaaggctg
gcatgacccacattgtccgggaggtggacaggcctggttctaaggtgaataagaaagaagttgtggaagcagttactgtggttgaaacacctcccatggt
aatagttggtattgttggctacgtccagactccccgtggtctgcgcagtttgaagaccatctttgctgagcacatcagtgatgaatgcaagaggcgcttt
tacaagaactggtacaaatccaagaagaaagcattcacaaagtactgcaagaaatggcatgatgacaagggcaaaaagcagctggagaaagactttgcca
gcatgaaaaattactgcccagtcatcagggttattgcccatacacagatgcgcctgcttcccctgcgccagaaaaaaatctcacctcatggaaaattcag
gtgaacggagggacagtaacctgaaaaagggtcgaccggggccccctggagaaaatttggaacaagcatggttggccaatcaacaagaattggttccggg
ccagggatgaaaaagatttgaaagtgaattgggggtccactaaaagagaaaaaggccttcaaaaaggggtgaataaagccccctgggggcacaaaaaaaa
gcctggcccccctcaaaaaatccaaaagaaggtctttctctaaagtgggggcctggtatttggtaaatttgtggaaatcctagcccccgttgtgggcccc
ttcttcaattaacccctcggcgttgggaaaacaaagggggatgatctccctcgctcgttcaanagggttccttatcagcaagaatttttataaaaatatt
ggttcccgcggcgatcc
3' EST: CF783096 [+]ttttttttttttttttaatttttagatgttatttttattaagagctcacagataaaactgtagtatctcagacaaacaggaacaaagttcatattcatgc
agtttcctcttttgccaagcggtccttcttgagtggtcccatgaaagccttcttttcctctgctgtctggaaacgaccatgcccaaacttggaggttgta
tcaataaatttaagatcaatcttctccagggcacggcgactggtttgcacaaggagggacttgcgcagtgtgagcactctcttcttggtcccaataacac
agcctttcaacatgacaaagtcgtttttcacctcaccatagtgaacaaacccacccagagggttgatgctcttgtctgacagatcgtagtctgttgaagc
gttgttcttgacaagtttaccatcctttgaatggtagccctggccaattttgtaaatcttcttattgatctctgtgcggtggtggtatcccttctgtcca
gcacgggctactgagaaggccacacgggcaggatgccaagctccaatacaagccaccttacgaagacctctatgagtcttacggggcagcttctttgtgt
gccagcggctagttacaccttttgtagccctttcctttagtgacaccaatcacatcaatcattttatccctgcccggaacactcctgtgaattgcaacct
gctgctcccatttctcacggggcccagtcgaactttctcagcttactgtaacctccgtttcacttggaatttcccatgaaggggagaatttttttctggc
cccagggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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