XB-CLONE-4354261
Clone Name: IMAGE:7026605
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Gene:
tom1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783101
[+]
aacgccctctaattcagctccgactctttctacgcagttagcaagaatcaacttgtcctctgggtctgtgagtggaaccctgcagtccctgggtggaacc
aagaaaccagatgatgattttgatatgtttgcactgaccaggggaagctctttggcagaccagcgcaaaagtgtaaagtatgaagaaccgcaagctacag
atggtcttgcaggggctctggatacccggcagcagaacactggagctatcccagctgcccaaaacaactttatggatgacattgagaagtggctgtcatc
aaatacgggtgaagagattgagggggaggatggtgtgaccactgaagagtttgataagtttctggaggaacgagcaaaggcagcagatcgcattccatca
ctgtcatcaccaggaacagagccccccagggcagcaccagtctcaaagagagacaaaaaggaagcggaggatgccctttttgccttgtgagtcctcccca
tggtgggtgagtgtgttgctccacgtggaaggaattattaaacgtctctatgcgtggcagtgatcatctcactgtttttttggagtctgctataaaaaat
tccttctgctgaaaactaacttttaatggggtacaagaaccttttgcaatctgggatgccctttcttatcccaacccatggaactgggggaaatctactt
tgttcttttctcaaggtcttcccccctttgggccggcctcaaatgtttctttcctgcgtcaatttcagctatttccaattccaactatagccaatggtaa
atattaaccatatgtccctcttttatcctaggctgaaaaaattaccgacacgtgggagacacttttttcaggcctatttgttgatctctaaagagaaggt
ttccaacaaaaaaaaactgtaataaggtcggggataaagacctccccgccccaaaa
3' EST: CF783100
[+]tttttttttttttttctgttttaacttagaattctttatttggatgcattgtccctgaggaagagttaaacacaaacagcctaggtttgcattacatgac
atttattttgctaatttctcttctgctttcatatcttctctgtcatattaaacatttacgtataggctacattttaaaatgaagcttcttggttgccttc
aagactatgttatttcaactcttctgctgccctttctcaggtacaggctcttaattcttaagattctttttaggatttctcagtcacttcctcgacccta
gaagagaccactttcccgtcgactacttcttcgatcaccgtcttcacctttttgatggagctcttgctttgttccttactgacgatggtaacagctttct
gtgtttcagtctgggaaaatctggtgtcttctccttctagcaggcgtctgtaggtggaaatttccatttccagccttgtcttggcatcgagcaacaactt
atactccaaagactggcgctcggcatctgaccgtaagttttgaaggtctaattctatttgcgtgaagatggcctgaaggtggttgaactcagctccatat
cgggcctcggtttctgccaaagtgccttccagtgctcctttcatgctcaagagtgactgaagttcaatctccaggctctgtattgtgcgcttcaagtctg
taatctgcgacttaaatgtctggagagcagctgtgttggtggcaacttctttgttgaagattttcgcctctggccctctgtacca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: