XB-CLONE-4354238
Clone Name: IMAGE:7026594
|
Gene:
krt7
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF783115
[+]
taattaatccccccccccccccgcgcatcagctccactcgctcctccagcggaggcgcagggctcggccgggcagggggaagcttcagcagctccagtct
gtccaacatcggtggcggcgtcagaaagagcgtctcttacggcgttagcagcggccgctccggtggcgcaggattcggtggcgctgggttcggtggcgca
ggattcggtggcgcaggattcggtggcgctgggttcggtggtggagtagcttattccggacctgggatccaggaggtgacaatcaaccagagcctgcttg
cccctctgaacctagagattgatcccaccattcagcatgtcagacaggaggagagagaacagatcaagaccctgaacaacaagtttgcgtctttcattga
taaggtcaggttcctggaacaacaaaacaaggttctggagacaaaatgggaactccttcagaaccagaaatccgccaaagccagcaacgttggccccttg
tttgatgcttacattgccaacctgagaaggcagctggatggtttgactggcgataagggcaaactggagggagaactgaagaacatgcaagatttggttg
aggacttcatgaacaagtatgaagatgagatcaacaagcgcacttccgctgaaaatgaatttgttgttctgaaaaaaggatgtggatgctgcccacatga
acaaattagagctggaggcccagatggagggctccgacaaatgagatcaactttcctgaagggcccttgtttgaagccggaactcccttgaagctgcaag
gaacaaattctcttgaacacttcaattccctcttgtccttttgggaaaaacaaccaaaacctccggggaaattgggaacggcaatcttttggccccaaaa
attacaaggggcccccatttatttagaaaaaatttttgtcgcaaaaaaagt
3' EST: CF783114
[+]tttttttttttttaaattgaaggaatttttattggagttgcttgtgtttctcaccagacataaaatccctcccacagaattcagatttccaccacaaaca
cagaccatatctaaaagagtggtaactaaaacagaattgttccatatactgggtgttttacaactgcaaacaaacattaaggaatcactgttctttgttt
tcccatctaagctgccagatttacatcagagacttactagaatttgttttacgttatatgttagcaccgtatatctcttttgttacctatcaagcctctt
ctatgggaaaacaaaacaaacacaaagatatctgcccagaaatactgctaattttttatgtatttgttttaatacaggcatgagatttccattgtgaact
gagcaatacaacttgcagcggtcgatactgtaaaaacgtgcccagacttatggcgaataggacacctagattcagcatacgattagagccagtcccactg
gtagaagggggagcagtgaaggagaaagatgcaagtaggcacttgtagacaggaatagggaactgctttcaagatttctgcaaacagccatatcctccng
ataataacaaacaacaaaatgcgggtgctagactacacacttggaaaggtgccaccaagatataggtataaggggtataacccactaagttaataccctt
accaacaccaacacattgtaaacagagcctgtttgaattatttgagggctatggctaatgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: