XB-CLONE-4354236
Clone Name: IMAGE:7026593
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Gene:
trmt13
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723021
[+]
taattaatcccccccccccccaatatatccttataatcggtgcttaggaatgtaatttgtatcccttgagtttttaaacctcctgtattacaaaacaatg
taccccctgcttaaaatatattagaataataatagaagtcatatctgaactgtgtgacctgtataaacccttggcctgcagctttgtgactttccgtgat
catggatatattctctgtatatttgtgttgagtgacttaatgtgatatttcctgcaggagcatgacagcaggagacgaattccatgccctttggacccca
agcacacagtgtatgaggatcagcttccaaagcaccttaagaaatgtaattccagagagaaacccaagcctgttttttatgttcaagatgtgaatgcagg
gtgcctgatcacagagacacctgataatcaggttggtatttagttaaaacaacccattcattctttcactgctgcattggaaacccctctttcctataaa
aatgttttgcaatttacaaatatcgcttatattccctctccaaagatgagcttttaggaactttaaaaagaaactgaaaacaagtgaactaattggccct
ggaaaccctcccaccatcataaataaaaaaactcctgtacactgggtccttttactttgaaagacccttgtaattgattcctatccaaattggaataaac
actgccattcccaaactattctccaaaaaaacacaggaccctcccatatttctggggtatactatgggaaaaaatctgtggggatttgtccccttttggg
atatccactcttgtttctataaaaagtggaccccgaccacacctttgtgtgagaatatttagaaccgctggaacaccaaaatatttttttttatttggca
caactctataaaatgaagatttcaacaatataaagacataaataaaaattttttacataanattcataccctattaaaatacccacttttattttttcca
tattcttaatttccac
3' EST: CF783116
[+]ctgagcaaacccagattgtccagatgacccagaatcgaggcctgctgcttgagatgcttggatgcagtgtctccatttgctggatcattcaaggcttcaa
gtaaagaccagtgacagagtttcttatctgctgctggagggtccaggccactagtcacttgtttcagcttctctgtaagttcctgaagctcatctttgga
gagggaactaagcgatatctgtaaattgcaaaacatttttataggaaagaggggcttccaatgcagcagtgaaagaatgaatgggttgttttaactaaat
accaacctgattatcaggtgtctctgtgatcaggcaccctgcattcacatcttgaacataaaaaacaggcttgggtttctctctggaattacatttctta
aggtgctttggaagctgatcctcatacactgtgtgcttggggtccaaagggcatggaattcgtctcctgctgtcatgctcctgcaggaaatatcacatta
agtcactcaacacaaatatacagagaatatatccatgatcacggaaagtcacaaagctgcaggccaagggtttatacaggtcacacagttcagatatgac
ttctatttatattccaatatattttaagcagggggtacattgttttgtaatacaagaagttttaaaaactcaaggggatacaaattacattcctaagcac
cgattataaggatatattggggggggggggggggaataattaaactcgaggggccacacaaaaccagaaattgggacttccggttctggaaaaaaactaa
cgtgccctttggaacctgggaaacttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: