XB-CLONE-4354233
Clone Name: IMAGE:7026592
|
Gene:
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF783118
[+]
taattaatcccccccccccccggtgagagtgactccttattgtgagactgactccttattgtgagactgactccttattgtgagactgactctggcttct
aaagttgggccccatagcgcttgtgaccaccgtggcaagaaatgatagtcacgtacaggcactagtggcagctgcccattctcgggggctcttgtcacct
gttgctgctatttttggtgagcatactcaaatcacttctccgagaattcctgtcttttttccttttgtgcctagcatctaacaccacaccccagcccctt
agtaatgatgtatctaatcctttccttaactaagttgccactaaccgcaataactacacaaacctcatgccagtgatccactgattatggctatgcatga
caaccctgatggttatatctctcgccgaaaacccataaaaacacactcgccctcactccctacccccactccatttgcccccttcctctcgcgcaccgat
tctccgcagatccatataataaccaaaacaattctcgtcttctgtcctgggactttattatgtcccatcatgtatcagcacaccccttgataccatcttc
aatatctaaatttcctcccgtacatctttacacagcccgtcctcttccgcataatttatctccgttctcttaattatcacacatccagaaacagctacag
taacattgctctaggttctttttattgatattcctttaccttcttgtctatataagtacacaaacacgcttctttgtatttaagcttctctcccatcatc
atcttatccttcattatattaagaggaaggcanatcagatagttcctctgtacggcatatgcagtgtatctatttctaattattgcctcacacgacctat
tgtcactagaggatcctctctctactccttcgtttgttggtgcggatgtatcccgaggtagtgatggagtgcccttctctcgcctccacacaccgacaaa
gaaacagaaatctagaaagagggccatatcatcccacagggctctcattattctcagaccatagtataactatcataatcaccactcagcgcagctatta
tttctn
3' EST: CF783117
[+]tttttttttttttttagagttaaagatcttttattgctttatgagacatttccataatcatttcttctttcttacgcaggtcttctctcttgtttcaggg
gaaatttctgttcatttgttcctttatctttcccgactgcccttggatttatgacactgttgaagaaacaaccttcccatctaccacctcttcgacaatg
gtttttactgttctggttctggttgttgttgttgatgatgattctgagctggactccagggaacttgcctttgacactgtggtcatctgtcccagttccc
catccagcaggcgcctgtatgtttcaatctccatctccagcctggtcttaatgtcaaggagctgcttgtactccatgttctgatgttccatgtcggacct
gatctgcagaagctgttcctctaggctgctgatagtgttctgaatctgtagaagttgcgctgaatagaagccttgtaattcattgaggttgccttcaact
gattgtttcattgccagctgggattgcagctcaatttccaagctctgaagtgaccgtttcagctcagtgatttcgctcttgctggcctgaacttgttcaa
ccccaactgaaatctctttcttcagctcaccgctcttctgggtgaaccacaactctgcatccctgcggttcttctcagccagtatttcatagtcagctct
catgttattcagaatgttggtcagatccacgcctgggaccgcgtccatctcaacactcactttttccggcgctctggctctttggcatg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: