XB-CLONE-4354228
Clone Name: IMAGE:7026590
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Gene:
gpx3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783121
[+]
taattaatcccccccccccccagattattcctgagtgctggaggaaaacagagataaatagagcaggggccgcctctagcccagcacaacacagagagct
gattcctgtacctcccaaagatgggggtcaaacttagggggctattaatgctcccatgcttcctggctgctctcatccatgcacaaaatgaaatggatca
gaaatcggtggattgctacagctccatcgatgggaccatctatgattatggggccactacattagacgggactcagttcatccctttcaaagcgtaccaa
gggaagtacatcctgtttgtgaatgtggccacatactgaggtctcaccatgcagtaccaagaattgaatgcactacaagaagaattaaaaaacaataact
ttgtcattttgggatttccttctaatcagttcgggatgcaggagcctggaaggaatgatgaaattcttttgggacttgagtatgtgcgtcccgggggaaa
attcgttccaaacttccaattatttgagaaaggagatattaatggacgaaaagaacagaaattctacactntttctcaagaactcccgcccccctgttgg
tgacaactttgggaagtgcaacaaataagactcatgtggggaaacccataaagggtcaacgaacgtcaaagtggaaaactttgaagaagttttctttgtg
ggggaccaaaaaccggccgccccagtgaaaacgcttgggctccccccgccacaccttggtcgcccccaagtggagaaaaaaaaaaattcaatgtccccca
catgaaaagtttaccgccccgggaaacaaccaaaaaccttcctttatggcctgggaattggggagccaaaaaaataaaaaccccttgtgcgagtttgtgt
ggcccatggttgggttccaaaccccacggaattggtatgtggtttggccgtggggaataaaaccaaaggtggtgttttggagaa
3' EST: CF783120
[+]ttttttttttttttttttgctgtttacaaacaaatattatttattgaatattggcacacattacattctattctagtgctcaagaggtaagttggaagct
aagggtaacactatgaagcctatcccagtctggcacagatgcctgaatgcactaagtagcatctgccccagcactggtttccagacaagtcccacacatg
agtgctttttgacttggccttcatacagatagcttatagattccctcagatctccaactgaccactcaaacacatagatgatcacagcaaacatacaatc
atggattgaacaacatggacacaactgcaatggttttatttttgctccaatccaagcataagaagtctttgtgttcccggctgtaacttcatgtaggaca
tgatctctcttctcacttgggcgacaggtgtgcgggggagccagcgtttcactgggcggccgtctggtcccacaagaaacttctcaaagttccacttgac
gtcgttgacctttatgggttcccacatgagtctatttgttgcacttccaaagttgtcaccaacaggggggcaggagttcttgagaaaagtgtagaatttc
tgttcttttcgtccattaatatctcccttctcaaataattggaagtttggaacgaattttcccccgggacgcacatactcaagtcccaaaagaatttcat
cattcctttcaggctcctgcatcccgaactgattannaaggaatcccanaatgacaaagttaatgttttttaattcctcttgtagtgcattcaattcttg
gtactgcatggtgagacctcagtatgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: