XB-CLONE-4354225
Clone Name: IMAGE:7026588
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Gene:
psmd6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783123
[+]
taattaatcccccccccccccgatcccttgccggtattgtttctgtgtctccgctgcctgtgccggtgccaagatgcctctggagaacctggaggaggag
gggctgcccaagaacccggacctccgcatcgcgcagctcaagttcctgctgagtctccccgagcaccgccgggaggtgccgctccgctccgagctcatgg
aggcggtgactagtaacaacatggctccttattatgagggtctgtgcaaacacctggactggacagtggacatggacctgttgaataaaatgaagaaggc
caacgaggaggagctgaagcggctggacgatgagctggaagatgcagagaagaatctgggggagagtgagatccgggacgctatgatggccagagcagag
tatctgtgtaggattggcgacaaggaaggggcccttactgcctttcggaagacctatgacaagactgtggcccttgggcaccgcttggatattgtgtttt
atctcctgaggatcggcctcttctatatggacaatgatctcattacacggaacaccgagaaagccagaagtcttatagaagaaggcggagactgggaccg
gcggaatcgcttgaaggtctatcagggcctgtactgcgtggctatcagagacttcaagcaagcagccgacctcttcctcgatacggtctccacaancacc
ctcctatgagctgatggactacaagaacctttgtgacatacacgggtgtacgttcagtatgatagcactcggaccgggccggacctgtataagagaaggt
ttatttaaagggggccaaaagatccctaaaaagtttgctggcacaaggctttaccccaccagtttcggggcaggatattcctgtgttcttccactgggta
cggagatgtgtcgctctatgtgggtggtctttccttttccaaatttcccctt
3' EST: CF783122
[+]catttctctcacgtagtaacggtagtgggaggcaaagagccagtccttcttaagctcctgttccacggcagccagggactggaagaagacaccatagcga
cactcgtacagtgagaacagatactgccggactgtgggtaagctgtgcagcacctctaggatctctgcccctttaataaccttctctctcaggtccggcc
ggtcgagtgctatcatactgacgtacaccgtgtatgtcacaaaggtcttgtagtccatcagctcataggaggtgaatgtggagaccgtatcgaggaagag
gtcggctgcttgcttgaagtctctgatagccacgcagtacaggccctgatagaccttcaagcgattccgccggtcccagtctccgccttcttctataaga
cttctggctttctcggtgttccgtgtaatgagatcattgtccatatagaagaggccgatcctcaggagataaaacacaatatccaagcggtgcccaaggg
ccacagtcttgtcataggtcttccgaaaggcagtaagggccccttccttgtcgccaatcctacacagatactctgctctggccatcatagcgtcccggat
ctcactctcccccagattcttctctgcatcttccagctcatcgtccagccgcttcagctcctcctcgttggccttcttcattttattcaacaggtccatg
tccactgtccagtccaggtgtttgcacagaccctcataataaggagccatgttgttactagtcaccgccctcatgagctcggaacggagcgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: