XB-CLONE-4354220
Clone Name: IMAGE:7026586
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Gene:
cct8
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080140 [+]
CTCTCTGAGTGCCGGTGATAGCGCGTTTGGTCGCTCCCACTCTCCGGCTCACCCACAGGTACCATCATGGCGCTGCACGTCCCCAAGGCCCCGGGCTTTG
CCCAGATGCTGAAGGAAGGAGCCAAGCACTATTCTGGATTGGAAGAAGCAGTGTTCCGGAATATCCAGGCCTGTAAAGAGCTCGCTCAGACTACTCGCAC
AGCGTATGGGCCGAATGGAATGAACAAGATGGTTATCAATCATTTGGAGAAGCTTTTTGTTACAAATGACGCTGCCACCATTATACGGGAGCTAGAGGTC
CAGCATCCGGCTGCCAAAATGGTTGTCATGGCTTCTCACATGCAAGAGCAGGAAGTTGGAGACGGCACTAATTTTGTTCTGGTCTTTGCTGGCGCTTTAC
TTGAGCTGGCAGAAGAGCTTCTGCGAATGGGACTGTCTGTATCCGAGGTGATCGAAGGCTATGAGAAAGCCTGTAAGAAAGCCTTGGAAATCCTCCCCGA
CCTGGTGTGCAGTTCCGCCAAGAACTTACGTGACATTGATGAAGTTGCGTCCCTTCTTCAGACTTCCATCATGAGCAAGCAGTATGGCAACGAGCTCTTC
CTTTCTAAGCTCATTGCCCAGGCATGTGTCTCCATCCTGCCCGAGTCTGGTCATTTTAATGTTGACAACATCAGAGTGTGCAAGATTTTGGGCTCAGGTA
TCTGTGCCTCTTCTGTCTTGCACGGCATGGTTTTCAAAAAGGAAGCAGAAGGCAATATTTCTTCTGTGAAAGATGCCAGAATCGCAATCTATTCCTGTCC
ATTCGACGGCATGATCACTGAAACTAAGGGCACCGTGCTGATAAACAGCGCGCAGGAACTGATGAACTTCAGCAAAGGCGAAGAGAATCTCATGGAAGAG
CAAGTAAAGGCAATCGCAGAAGCAGGGGCCACTGTTCTTGTGACAGGGGGCAAAGTTGCTGACATGGCTCTCCATTACGCAAACAAGTACAACCTCATGG
TAGTAAGGTTAAACTCCAAATGGGATCTTAGAAGACTGTGCAAAACTGTGTGTGGAACTGCCCTTCCCAGGCTGACCCCTCCTACAGCAGAGGAAATGGG
GCGCTGTGACAGTGTTTACTTGTCAGAAGTTGGGGATACACAGGTTGTTGTGTTCAAGCACGATAAGGAAGACGGCGCCATTGCTACGGTTGTGATCCGT
GGCTCTACAGACAATCTCATGGACGATGTGGAACGAGCAGTAGATGACGCCGTGAATACTTTCAAAATCCTCACAAGGGACAAGCGCCTTGTGCCTGGAG
GCGGAGCCACTGAAGTTGAGTTGGCAAAACAGATCACATCTTATGGAGAGACCTGCCCTGGGCTTGACCAGTACGCCATTAAAAAGTTTGCCGAAGCATT
TGAGTCCATTCCAAGAGCATTGGCGGAAAATTCTGGTGTCAAGGCCAATGAGATCATCTCCAAACTCTACGCAATGCACCAGGAAGGCAACAAGAACGTG
GGCTTTGACATTGAGGCGGAGAGTGCGGCAGTGAAAGACATGATGGAAAGTAACATCCTGGACACGTATCTAGGGAAATACTGGGGGATAAAGTTGGCTA
CAAATGCAGCGATCACTGTACTTAGGGTTGACCAGATTATAATGGCAAAGGCAGCTGGTGGACCAAGAGCCCCCAAACAGCAAGGACACTGGGATAAGGA
TGATTGGCAAGATGAACCAGACAAATCTTAGGGCTCTATTATCATGGCAAAACCAGCAGGCGGCCCAAAAGCACCCACGGGAAAGAAGGACTGGGACGAT
GACCAAAACGACTGAACACCGCCTTTCTGAATCCGTTGGTCTAATCAGTGTGTGCAGTACAGATTACCGAGTCGCTTTTTTTTTTTTTTTGCAATTTGTT
CACTTACCCTTAAGTTAAATAATACTGTATAATAAACATTCAGTTTCTGACAAAAAAAAAAAAAAAA
5' EST: CF783126 [+]taattaatcccccccccccccctctctgagtgccggtgatagcgcgtttggtcgctcccactctccggctcacccacaggtaccatcatggcgctgcacg
tccccaaggccccgggctttgcccagatgctgaaggaaggagccaagcactattctggattggaagaagcagtgttccggaatatccaggcctgtaaaga
gctcgctcagactactcgcacagcgtatgggccgaatggaatgaacaagatggttatcaatcatttggagaagctttttgttacaaatgacgctgccacc
attatacgggagctagaggtccagcatccggctgccaaaatggttgtcatggcttctcacatgcaagagcaggaagttggagacggcactaattttgttc
tggtctttgctggcgctttacttgagctggcagaagagcttctgcgaatgggactgtctgtatccgaggtgatcgaaggctatgagaaagcctgtaagaa
agccttggaaatcctccccgacctggtgtgcagttccgccaagaacttacgtgacattgatgaagttgcgtcccttcttcagacttccatcatgagcaag
cagtatggcaacgagctcttcctttctaagctcattgcccaggcatgtgtctccatcctgcccgagtctggtcattttaatgttgacaacatcagagtgt
gcaagattttgggctcangtatctgtgcctcttctgtcttgcacggcatggttttcaaaaaggaagcagaaggcaatatttcttctgtgaaagatgccag
aatcgcaatctaatccctgtccattcgacggcatgatcactgaaaactaagggcacccgtgctgaataaacagcgcgcag
3' EST: CF783125 [+]tttttttttttttttgtcagaaactgaatgtttattatacagtattatttaacttaagggtaagtgaacaaattgcaaaaaaaaaaaaaaagcgactcgg
taatctgtactgcacacactgattagaccaacggattcagaaaggcggtgttcagtcgttttggtcatcgtcccagtccttctttcccgtgggtgctttt
gggccgcctgctggttttgccatgataatagagccctaagatttgtctggttcatcttgccaatcatccttatcccagtgtccttgctgtttgggggctc
ttggtccaccagctgcctttgccattataatctggtcaaccctaagtacagtgatcgctgcatttgtagccaactttatcccccagtatttccctagata
cgtgtccaggatgttactttccatcatgtctttcactgccgcactctccgcctcaatgtcaaagcccacgttcttgttgccttcctggtgcattgcgtag
agtttggagatgatctcattggccttgacaccagaattttccgccaatgctcttggaatggactcaaatgcttcggcaaactttttaatggcgtactggt
caagcccagggcaggtctctccataagatgtgatctgttttgccaactcaacttcagtggctccgcctccaggcacaaggcgcttgtccccttgtgaaga
ttttgaaagtattcacgggcgtcatctactgctcgttccacatccgtccatgagatttgtctgtanaagccccggatcacaaccgtaaccaatggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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