XB-CLONE-4354218
Clone Name: IMAGE:7026585
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Gene:
chpt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723024 [+]
taattaatccccccccccccccccttgggtctggctgcggggctggcacggaggatgatgccccacctgtatatacaggagcccctgagcgcccaccaac
tgaagcggctggaggagcacaaatacagcgcgtctggccgctctcttgtggagccccctatgcaagtgtattggaactggctggtggagaaggtccccct
atggctggctcccaatactatcactatggtgggactgctgctcaacgtcatctccacacttatacttgtctgttattgtcccacagcaactgaagaggct
ccattttggacatacctgctttgtgctatagggctttttgtttatcaatcattggatgcaattgatggcaaacaggctcggaggacaaatagcagctcgc
cgcttggtgaaatgttcgatcatggatgcgactcaatctctatagtatttgtttctctggggacaatagctgctgtccggcttggaaccctccctggatg
gatgttctactgttgctttgtgagcatgttcatgttttattgtgctcagtggcagacctacgtatgtggcactctgaagtttggcgtaattgatgtcact
gaactccagctttctgttacagcaatgtttgtcatgacagcttttttttggcgcagcattatgggattatgagatccccctcactggcctacctatgaaa
acattgccacttctaagaataataggaggaacgggtttattcctgcagtaactattttagagtcattctcagtgggaagaatggggaaaaaatggatcca
cagtttgcagggaactaggggtgctattcccccgggccttacattattgggacttgtttctcctactggggcataaatggatttaccc
3' EST: CF783127 [+]tctttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttccccccaaaaaaaaaaaatttttttacccaccggg
gattaaaaaaaaaaaaaccttccccccccccctgttttaaaggggcttttggggaaaaaaaaaaaaacccccccccaaaacgggggggtgggggggcccc
ttaaaaatcccccccctttaaaaaaaaaccgccaagggggcccccaaaaaaaaaaaaaaattttttttgtttttccctttttggggaaaaaggggccaaa
accccccctgttaaaaacctttcccaaaaaaaacccccttaaaaaaaggggggaaaaggaacccccccccttttttttaaccaccaaaattttaaaggcc
cccccaaaaaaaaaaaaaaaaaatttgccccaattaaaagggggggggggataaaaaaaaatttagggggttcctttaaaaaccccccccccccaagggg
gaaaaaaaaaaaaaaaagggggccccttttttttcccaacccctggggggggtttttcccaatgggggcccaaaatccctggaaaaaaaaaaaaaaggtc
ccccggaaaaaccctttccccctccaaaaggggccccggggggtctaaataagccccccccctcaataatggggcgcaaaaaaaaaaaccccccccaaag
ggggggggccccataaaaaccgggggggggcccaaaatctttttgggggaaaaaaacccccccgggcccaaaaaaccggggaaaatatattttccaaata
aac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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