XB-CLONE-4354213
Clone Name: IMAGE:7026583
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Gene:
pgam2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723025 [+]
taattaatcccccccccccccggaacatcacagggaccagacttcaggctgaagatcattctgcccaataaacttcagtaaacgcctatacaggtagggc
tgttctatccaacaagtcagtcttatactagtcatctgtaattgcttgtagccaccatgcctcaccgtctggtaattgtacgccacggggaaagctcatg
gaatcaggagaacagattctgtggatggttcgatgccgacctgagtgagaaaggggcagaggaggctcgcagaggagctcaggcaataaaggaggccgga
atggagtttgacatctgctacacctcagtgctgaagagggcagtgcgtacactatggtacatcttggatggaatagaccaaatgtggctgccagtggtca
ggacctggagactcaatgagcgccactatgggggactgacaggcctcaacaaggcagagacggcggagaaacatggggaagagcaggtgaagatatggag
aagatcttttgacatcccaccaccagtcatgggagaagatcattcatactataagcttataagtaaggaccgtcggtacaaagatctcactcagaaagag
ctgccatcctgtgagagcctgaaggacaccattgcccgtgccctcccattttggaacgaagtgattgccccccagatcctggcaggaaagcgcgtaatga
tagcggcacatggaaacagcttgagaggcattgtcaagcacctgggaagggatggtcccatgctgcccattatggaactcaatctaccccactgggaatt
cccaattgttttatgaactaagatgaaaaacctggaaccaaatccaaaccccattgagcttttcttttgggggaataaaaga
3' EST: CF783130 [+]tttttttttttttttggttttatttctttattttggataaaaatacagaattagtaaattggtgactgtgaaaagaacatttaaaatcgttttaaaatgc
tcacaaaaaaaaaaaataactggatgactgcatgtacatatatgatcagtaggtcacgcaatgtgaatttactctcatcacaaggtgagtgagtgtgctt
gtgtgatcactacatcatggggagtgcatgcacactacctgtatgataacaaggtcaccaagtcagcatgtatgtgagctcataatgtgacccgtgagta
ttaagacactgggtattatatatagcatttgtacttacactagaggagcattctcccctctttgtcccaatgttatttcccagaatcttattttccatgc
atgaaaacaggcttataagaataacatggggactaggaagtttttgtgtgtcctactactactctcctttatcctctctttctataacccaaacccctat
ttgcccatctattttttgacctttccctgagctgccactgcctccatggcttttcgtacagtttcctcatccccaaggaagctcatgggtttgattggct
tcaggttatcatccaattcaaaaaacatgggaattccagtgggtaaaatgaattcccctaatggcagcatccgaacatcccatcccggtggcttgacaaa
gccctctcaagctggtttccaggtgccccgctatcattaacccgcttttcttggccaggaactggggggggcaaaccagttcctttccaaaaatg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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