XB-CLONE-4354205
Clone Name: IMAGE:7026580
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Gene:
cand1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077698
[+]
ATCGCTTATCCAATCGGAACAACAGGAACGGCCCCACGCCGCCTGCTTTCGAGTATACGCCGTAAACTTTGACTTGTTGGGCTCTCGGGAGGCTGGCTGC
CATCCTGCCTGTAGTGCTGTGCTCCGCCCCACAGACGGGGCTGTATGTGAGACAGGCTGGTTAGCGCGGCCTCCAAATCGGCTCCGTAACGAGTGCGCGC
ACCGTAGGCCTCCCGAGCGCCTGAAAGGAGACGGGTGCGAGCCGGACTGCTGGAAGTCGTGGCCCCAAAGCAAAGGCTCTTTTTTTCCACCCGAGGCTAC
GGAAAGTGCCGCAGCCATCATGGCGAGCGCCTCGTACCACATCTCCAATTTGCTGGAGAAGATGACATCCAGTGACAAGGACTTCAGATTCATGGCAACT
AATGACTTGATGACAGAGCTTCAAAAGGACTCCATAAAGCTAGATGATGACAGTGAAAGGAAAGTAGTGAAAATGATATTGAAGCTGTTAGAAGATAAGA
ATGGGGAAGTTCAAAATCTAGCTGTGAAATGCCTTGGTCCACTGGTAAGCAAAGTTAAAGAATATCAAGTGGAGACAATTGTAGACACCCTTTGTACCAA
CATGCTTTCGGACAAGGAACAGCTACGTGATATATCAAGCATTGGGCTTAAGACAGTAATTGGAGAACTTCCACCAGCTTCTAGTGGATCTGCTCTAGCT
GCAAATGTTTGCAAAAAAATTACAGGCCGACTTACAAGCGCTATTGCAAAGCAGGAAGATGTGTCTGTCCAGTTAGAAGCTTTGGACATTATGGCTGATA
TGCTTAGAAAAAAAAAGAAAGAAAGAACGAAAAAAAAAAAAAAAA
5' EST: CF783135
[+]taattaatcccccccccccccatcgcttatccaatcggaacaacaggaacggccccacgccgcctgctttcgagtatacgccgtaaactttgacttgttg
ggctctcgggaggctggctgccatcctgcctgtagtgctgtgctccgccccacagacggggctgtatgtgagacaggctggttagcgcggcctccaaatc
ggctccgtaacgagtgcgcgcaccgtaggcctcccgagcgcctgaaaggagacgggtgcgagccggactgctggaagtcgtggccccaaagcaaaggctc
tttttttccacccgaggctacggaaagtgccgcagccatcatggcgagcgcctcgtaccacatctccaatttgctggagaagatgacatccagtgacaag
gacttcagattcatggcaactaatgacttgatgacagagcttcaaaaggactccataaagctagatgatgacagtgaaaggaaagtagtgaaaatgatat
tgaagctgttagaagataagaatggggaagttcaaaatctagctgtgaaatgccttggtccactggtaagcaaagttaaagaatatcaagtggagacaat
tgtagacaccctttgtaccaacatgctttcggacaaggaacagctacgtgatatatcaagcattgggcttaagacagtaatttggagaactttcaccagc
nttctagtggatctgctctagctggcaatgttttgcaaaaaaatacaggcccgacttacaagcgctattgcaaaccaggaagatgtgtctgtccagttta
aaaactttgggacattatgggctgataatgcttngaaaaaaaaaagaaggaaagaccgaaac
3' EST: CF783134
[+]tttttttttttttttcgttctttctttctttttttttctaagcatatcagccataatgtccaaagcttctaactggacagacacatcttcctgctttgca
atagcgcttgtaagtcggcctgtaatttttttgcaaacatttgcagctagagcagatccactagaagctggtggaagttctccaattactgtcttaagcc
caatgcttgatatatcacgtagctgttccttgtccgaaagcatgttggtacaaagggtgtctacaattgtctccacttgatattctttaactttgcttac
cagtggaccaaggcatttcacagctagattttgaacttccccattcttatcttctaacagcttcaatatcattttcactactttcctttcactgtcatca
tctagctttatggagtccttttgaagctctgtcatcaagtcattagttgccatgaatctgaagtccttgtcactggatgtcatcttctccagcaaattgg
agatgtggtacgaggcgctcgccatgatggctgcggcactttccgtagcctcgggtggaaaaaaagagcctttgctttggggccacgacttccagcagtc
cgggctcgcacccgtctcctttcaagcgctcgggaggcctacggtgcgcgcactcgttacggagccgatttgggagggcgcgctaaccagcctgtctcca
aaacagcccccgtctgtgggggcggagcacagcccttccgggcaggaatggcacccagccttcccgagagcccaaaaagtcaaaagtttaccgggtta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: