XB-CLONE-4354198
Clone Name: IMAGE:7026576
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Gene:
rpl9
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783139
[+]
taattaatcccccccccccccccccccgctttcctctgtcagctgcgagaatgaagaccattctcagcaaccagattgttgacatcccagagaatgttga
catctctctgaagggccgtacagttactgtaaaggggcccagaggtgtcctgcggaaaaactttaaccacatcaatgttgagctgtgtcttctgggcaag
aaaaagaggaggctccgggttgacaaatggtggggtaacagaaaagaactggccacagtgcgcaccatctgcagtcatgtgcaaaacatggtgaaaggag
tcacactgggctttcgttacaaaatgagatctgtgtatgctcactttcccatcaacgttgtcattcaggagaatggatctcttgtggaaataagaaactt
cttgggtgaaaagtatatccgcagggttcgcatgagatcaggtgttgcatgcgccttatcccaagcccaaaaagatgagctgattcttgaaggcgatgac
attgaactttgtatcaaactctgctgccttgatccagcaagccaacccagtaaaagaataagggatgatcagaaaatctctgggatgggttcctaccgta
tctcgaaaaggggttcaagttactgcagggtgtgtaagaataaaaatgccctggcagaaaaggctgggtttccccgtgggctggttcccaagaatatata
agggccccttttgtttctaaatgtaaaatangaaaaaagagggggatcctcccaacggttccctggaagggtgggccccccctgcgcggtgggtggggag
ccatttgggtctgtagggtccactaacttttattgtttcttatgtatcaactataataagtgcggatttaacaatattttaaaggaccaagatagccaac
ccacccgaaaaaatttactactaaaaaataaacccaaaaaattattcactaccacacgagagaaattatcaggatcgaggggggggttattcgtggcccc
tataacgcctcaccactcaag
3' EST: CF783138
[+]tttttttttttttttagataaagggactttatttttggaacagcacaggaaccagacttttgcaggcttttattcttcaacctgctgtactgtacccttt
tcggatacgtagataccatccaagaattttctgatatccttattctttactgtggtggcttgctggatcaaggcagcagagtttgatacaagttcaatgt
cattgccttcaagaatcagctcatctttctgggcttgggataaggcgcatgcaacacctgatctcatgcgaaccctgcggatatacttttcacccaagaa
gtttcttatttccacaagagatccattctcctgaatgacaacgttgatgggaaagtgagcatacacagatctcattttgtaacgaaagcccagtgtgact
cctttcaccatgttttgcacatgactgcagatggtgcgcactgtggccagttcttttctgttaccccaccctttgtcaacccggagcctcctcttcttct
tgcccaaaagacacagctcaacattgatgtggttaaagtttttccgcaggacacctctgggccccttttacgtaactgtacggccctttagaaaaatgtc
aacatttctcggggaatgtccaacattccggtttgctgaaaaaagggtcttcatttctcccagctgaacaaaagaaaaccgggggggggggggggggggg
ggatatgtctaagaggttattttaccgcgcctaaaaatgtattataaaaattacaacatgactgaggcatcagtcttaacattctatcgtcgtggctgta
ccccgagagcaatctctggttagaataata
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: