XB-CLONE-4354193
Clone Name: IMAGE:7026574
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Gene:
XB5733233
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783142 [+]
taattaatccccccccccccctcacactcattcctctgggactcggccagagaaagaaacacaaagcacattctcagagaggaaatatttacaaaccaaa
ggggacggaaatcttctacacaaacctccagtggaactgcagagagagggagaggagccttccagccccaaggacttgctagtctgtgcttgttactttc
ttccctatcagctcttgctgggtttgcgtggacttgaattgtgaaaagaagcaaagttcccagtttgggatggctgaatgtcagtgtgttctctggtgca
tcttctgggtcttggtcctgatcttcattggctggccagtcagcattttccttggaggcctgtatgggtttctgtctcctcttaccacctgcataggatt
ggacaagttcacagaccttctgctccaaggagtcaacattggcaggacctgtgctgaaaatgcccgtaccggcaaatcattgtgctagaggaaggagatg
gactctctgcaactaaatcagaagcttcatctctagccaaaatcacattccagcctctgcttgcttctgcagggcacaggtcttcccagctggaaagcca
caaggttattcccacacaatgaaggtgctgtatctcccaagctttcttgtttaagaaggacaaaagctctcactgggaaattttttttcagaccagttac
taaattgggcttggttaatatgtccatgtgtcaggatcttggcaccatttttataatccatggacccggtatttaataaacatagtttggttacccccct
attttggggaaataaaaaaattccagagtggttttcctttttcgcgctcagtctaggctattgtcccataagaggagaaagagaatgtaatctggaagaa
gaggagggggttccccccctatgtttttcctaaaaaaaggcgggccgccgccccacacccgccgggtggagagaaaaacttttgtgggcccgc
3' EST: CF783141 [+]tttttttttttttttttttttttttttttttttttttttttttaatgaaacatctgatttattattccatatatgagtacaaactagttataatacgtgt
catgatataaaatgtgccaaatctgacacatgacatataacaagccaattagtaactgtctgaaaaaaatcttccagtgagagctttgttccttctaaac
aagaagcttgggaaatacagcaccttcattgtgtgggaataaccttgtggctttccagctgggaaaacctgtgccctgcacaagcaagcacaggctggaa
tgtgattttggctaaagatgaagcttctgatttaattgcagaaagtccatctccttcctctagcaacaatgatttgccggtacgggcattttcagcacag
gtcctgccaatgttaactccttgggaagcaaaaagtctgtaaacttgtcccatcctttggaggtgggaaaaggagaaaaaaaccctacagggccctccca
ggaaaatggtgaattggggccagcccaatagagatccggggcccagaccccccaaaagatggcccgcccaaaaaccacacctgaacatttaggcctattc
caaaaacaggggagaaacttttgctctcgttatataaagatattccaggtccagcacacaccccccggtataaagttttaagggggaacataatgtccac
aaacaccgaggattgtcaaatctcccttggggggcttggaaaaaggccctgtctcctttctgatttttcggagatcccctgtggatggtttttgggataa
aaaaaatttttcggccccccttttgagagtttgtaataataatatccctccccaccaaaaaattagtgcgtcttgcg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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