XB-CLONE-4354184
Clone Name: IMAGE:7026569
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Gene:
psmb2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783148 [+]
taattaatcccccccccccccatgttaccggcacccatcatgttaccggcgatatctgatgacgttagtagagcgcgcccattcccggccttgtgctgat
ttgctgtgtaacgtcccgaggccgtaattagagactcataactcggggaaccaacagcggcgggaatatggagtatctgattggcattcagggcaatgac
ttcgtacttgtggctgcggacacagtgtgtgccaacagcatcattaagatgaagcacgatgtggacaagatgtttaagatgagcgagaagatcctgctgc
tgtgtgtaggagaggcaggtgacacagtccagtttgcagagtacattcaaaagaacgtgcagttgtacaaaatgaggaacggctatgagttgtctcctac
tgcagccgcaaacttcacacggagaaatctggctgactatcttcgcagcaggaccccttaccatgttcatctgctgcttgctgggtatgaatgacatgac
ggaccctcgttgtattacatggattacctagcagctctagcaaaaacgcgttttgctgcacatggctacggggcttactaaccctcagtattctggatcg
gctctacaacccggacctgaacaaaagaggaccccgttaaacctacctaaagaaatgggttttgcaaaacctccgaaaaccgctttttatccttgaagcc
tgcccttttcttttcaaaggggaagggtgcctttcgataaaggggaggggggatcccctgggaactgtggggaaacgagaaatttctcgggcctttcagg
ggcctcttttataacccttt
3' EST: CF783147 [+]tttttttttttttttgggagtcccacatgcaaatgcacagggtaatttatttgtttcatacagtggtacaaattaaccttaatatataaaaagaaaaagg
aggaggcgagtcagggagaggtaaggttaaaggcctgaagcaggaatcgtctccaagtcatggatcccatccttatcgatgaccctcactgtgaaggaag
gcaggctcaggataaagcgcttctggagctctgctatacatttctttagtagttctactgcgtcctctcttgtcaggtctggtttgtagtagcgatccag
aatactgagggttaggtaagccccgtagccatgtgcagcaaaacgcgtttttgctagagctgctaggtaatccatgtaatacaacgagggtccgtcatgt
tcatcatacccagcaagcagcagattgacatggtaaggggtcctgctgcgaagatagtcagccagatttctccgtgtgaagtttgcggctgcagtaggag
acaactcatagccgttcctcattttgtacaactgcacgttcttttgaatgtactctgcaaactggactgtgtcacctgcctctcctacacacagcagcag
gatcttctcgctcatcttaaacatcttgtccacatcgtgcttcatcttaatgatgctgttggcacacactgtgtccgcagccacaagtacgaagtcattg
ccctgaatgccaatcagatactccatattccccgcccgctgttggttcccccgagtttttgaagtctctaattacgggccctcggggacgtt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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