XB-CLONE-4354181
Clone Name: IMAGE:7026568
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Gene:
rpl3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783150
[+]
taattaatcccccccccccccaactccctctttcagttcggtcagggcttgagatgtctcaccgaaagttttcagccccccgacatggttccctgggctt
cttgccccgcaagcgttccaagagacatcgtggcaaagtgaagagcttccctaaggatgaccagagcaagcccattcacctgactgccttcctgggctac
aaggctggcatgacccacattgtccgggaggtggacaggcctggttctaaggtgaataagaaagaagttgtggaagcagttactgtggttgaaacacctc
ccatggtaatagttggtattgttggctacgtccagactccccgtggtctgcgcagtttgaagaccatctttgctgagcacatcagtgatgaatgcaagag
gcgcttttacaagaactggtacaaatccaagaagaaggcattcacaaagtactgcaagaaatggcaggatgacgagggcaagaagcagctggagaaggac
tttgccagcatgaaaaagtactgccaagtcatcagggttattgcccatacacagatgcgcctgcttcccctgcgccagaagaaatctcacctcatggaaa
ttcaagtgaacggaggtacagtagctgagaaggtcgactgggcccgtgagaaattggagcaacaggttgcaatcacaggagtgttcgggcaggatgaaat
gattgatgtgattggtgtcactaaaggaaagggctacaaaggtgtaactagccgctggcacacaaagaaactgccccgnaaagactcataaaaggtcttt
cgtaaagtgggcttggtattgggaacttgggcattcctgccccgtgggggccctttctcaatataacccggtggtgggaacaaaaaagggaaaccct
3' EST: CF783149
[+]ttttttttttttttgtatttttagatgttatttttattaagagctcacagataaaactgtagtatctcagacaaacaggaacaaagttcatattcatgca
gtttcctcttttgccaagcggtccttcttgagtggtcccatgaaagccttcttttcctctgctgtctggaaacgaccatgcccaaacttggaggttgtat
caataaatttaagatcaatcttctccagggcacggcgactggtttgcacaaggagggacttgcgcagtgtgagcactctcttcttggtcccaataacaca
gcctttcaacatgacaaagtcgtttttcacctcaccatagtgaacaaacccacccagagggttgatgctcttgtctgacagatcgtagtctgttgaagcg
ttgttcttgacaagtttaccatcctttgaatggtagccctggccaattttgtaaatcttcttattgatctctgtgcggtggtggtatcccttctgtccag
cacgggctactgagaaggccacacgggcaggatgccaagctccaatacaagccaccttacgaagacctctatgagtcttacggggcagcttctttgtgtg
ccagcggctagttacacctttgtagccctttcctttagtgacaccaatcacatcaatcatttcatcctgccccgaacactcctgtgattgcaaacctgct
gctcccatttctcacgggggccagttcgacctttctcacctactggtaccttccgttcccctggaatttccatgaaggggaaaatttttcctggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: