XB-CLONE-4354178
Clone Name: IMAGE:7026567
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Gene:
best2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783152 [+]
taattaatcccccccccccccggtgagagtgactccttattgtgagactgactccttattgtgagactgactccttattgtgagactgactctggcttct
aaggttgggccccatagcgctgtgaccctccgtgggaagacatgacagtcacgtacaggcacgagtggcaaatgcccgttttgggggcttttataagctg
ttgctgctatggagggggagcatatacaaattactttacaaggaattcctggcttttttcctcatgtacctggcgcttagcatcatatacaggttctttc
tgaatgaggaacagaaactttactttgacaaagtggccatttactgtaataactacgccaacctcatccctgtgtcctttgtgctgggtttttatgtcaa
ccaggtggtaaatcgctggtggaaccagtatctctctttgcctttccccgaccgtgtcatgtgcgccatctccgggactgtgcatggatctgatgaaacg
gggcgtctgtaccgccggactcttatgcgctactgcagcctatcagggctattgattctgcgctctgttagtaccgctgccttcaaaagattccctacaa
tagaccatgttgtagaagctgggttcatgactcgcctggagagaaagaagtttgagaacctccagtcatcctataacaagtactgggtttccgtgcgtgt
ggttctngtaccntggcgtcncaggcgcggaancgagggggaggatccgaagatgaccatttcctttaannatgctgatgggaggagcttgaacaccttt
ccgggggggaacttgcggcatggctccttc
3' EST: CF783151 [+]ggaactggcgtccaatcagacaggtcaggaagaagctgtacaccgcaattgtcacaacctgagtataaaccagggggacgctgatccagtcatagtggaa
gagcatgccgcagttcccccggaaggtgttcagctcctccatcagcattttaaaggaatggtcatctcggatcctcccctcgctccgcgcctgcgacgcc
aggttacagaaccacacgcacggaacccagtacttgttataggatgactggaggttctcaaacttctttctctccaggcgagtcatgaacccagcttcta
caacatggtctattgtagggaatcttttgaaggcagcggtactaacagagcgcagaatcaatagccctgataggctgcagtagcgcataagagtccggcg
gtacagacgccccgtttcatcagatccatgcacagtcccggagatggcgcacatgacacggtcggggaaaggcaaagagagatactggttccaccagcga
tttaccacctggttgacataaaaacccagcacaaaggacacagggatgaggttggcgtagttattacagtaaatggccactttgtcaaagtaaagtttct
gttcctcattcagaaagaacctgtatatgatgctaagcgccaggtacatgaggaaaaaagccaggaattccttgtaaagtaatttgtatatgctccccct
ccatagcagcaacagcttataaaagcccccaaaacgggcatttgccactcgtgcctgtacgtgactgtcatgtcnttcccacgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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