XB-CLONE-4354168
Clone Name: IMAGE:7026562
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Gene:
myl6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783158 [+]
taattaatccccccccccccccgacattccggaagggagaagacgcgtcaagatgtgcgactactccgacgaccagattgccgactacaaggagtccttc
caactttttgaccgtgtcggcgatggcaaaatcctcttcggccagtgtggggacgtcatgcgggcactggggcagaaccccactaatgctgaggtgatga
aggttttgggaaacccaaagcctgaagacatgaacatcaagacacttgacttcgagcaattcctccccatgatgcaaacagttgccaagaacagggatgt
acctggattggaggacagcatagaagggctaagagtcttcgacaaggagggaaatggcacagttatgggctccgagttgcgtcatgtcctggtttccctc
ggagagaagatgacagatgatgaagtagagacccttttaagtaatcatgaggatgctaatggttgcattaattatgaagaacttatccgggcgatattga
acggctgaagcttctcctttagtttacctctgcccagtggggatgaagtgtgggggggaagttagttagcaatttattcaagcccatcactagagaggaa
gacaggctatatagtaagtgccttttctgcttcctccttatcccatcagtcacccccttcttcttgcaagtggcaaaattcgtattcatttttttctgta
catcacaaggggaggcccacagctacagcttaaatgaacatcggtagaaattgtggcaaaataatttttcatatcaccccccccccccccatgcttcctt
aaagccggtatggggcaaaaaaaaaataaatttattccaaatttccaattttttccccctcccccacatcaaaataaaaccggt
3' EST: CF783157 [+]tttttttttttttttctttttttttttttttcttggaaaaagtcactcagtttatttgatgttggagaggggaaaaaatagaaatttgaaataatttatc
tttctctgcacactacagcattaaggaagcatgggggggggggggtgatatgaaaaattattttgccacaatttctaccgatgttcatttaagctgtagc
tgtgggcctcccttgtgatgttacagaaaaaatgaatacgaattttgccacttgcaaaaaaaagggggtgactgatgggataaggaggaagcaaaaaagg
cacttactatatagcctgtcttcctctctagtgatgggcttgaataaattgctaactaacttcccccccacacttcatccccactgggcagaggtaaact
aaaggagaagcttcagccgttcaatatcgcccggataagttcttcataattaatgcaaccattagcatcctcatgattacttaaaagggtctctacttca
tcatctgtcatcttctctccgagggaaaccaggacatgacgcaactcggaacccataactgtgccatttccctccttgtcgaaaactcttagcccttcta
tgctggtcctccaatccagggtacatccctggttcttggcaactgttttgcatcatggggaaggaaattgctccaaaatcaaagtggtctggaggttcat
gtccttcaggcctttggggtttcccaaaaactttcatcccccttcaaattttagggggggttctgcccccaatgcccccccaataacatcccccaccatg
ggcccaaaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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