XB-CLONE-4354165
Clone Name: IMAGE:7026561
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Gene:
ran
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783160 [+]
taattaatcccccccccccccggctgctttacagacgcttctggaaggctgacaaacataccatggcagcccaaggagaacctcaagtgcagtttaagct
tgttctggttggggatggtggtaccggtaaaacaacctttgtgaaacgtcacttgactggtgaatttgagaagaaatatgtagccacacttggtgttgaa
gttcatcccctggttttccacactaacagagggccaatcaagtttaacgtgtgggatacagctggtcaagaaaagtttggtggtctcagagatggatatt
atatccaggcccagtgtgccattatcatgtttgatgttacatcaagagtcacatacaagaatgtaccaaactggcatagagatcttgtaagagtgtgtga
aaacatcccaatagtcttgtgtggtaataaagtggacataaaggacagaaaagttaaagccaaatcaattgtcttccataggaagaagaatcttcagtac
tatgatatttctgctaaaagtaactacaattttgagaagcccttcctttggcttgcaaggaaacttataggtgaccccaatcttgagtttgtagccatgc
cagcccttgccccaccagaagttgtaatggatccggcattggcagcacagtatgagcaagatttacagcatgctcaggccactgcactgccagatgaaga
tgatgatctgtgaggggaatgaggctggagcccagcgtcagaagtctagttttataggcaactgtcctgtgatgtggtgcancgtgtttgccactctatt
atatagctgagcagaacatgtgcttaatctttttggggatgcaaaaggaaatgaatgggcttcggagtgaatgttggcaattcaaaaaaaa
3' EST: CF783159 [+]tttttttttttttttaaggtttttcttctttattatattgtggttttataatagggtcactggtataaagcaaattgttttaataagggtgggtcagtgc
acacaatgatttggagatgggggttgtcattaaaggataagtaaacctttactgtaaaattgctgagtgctattctaggcacttttgcaatttaattttt
taaattccaagatattaacatatgtttttactgtggatgcaagaaaagttgaggtttctaatgttttttttctacccaacaagaacaaatcagcctttta
taacaacatttattcaaattaatagcacatatatcccttaatatcttggaattttaaaggtttagccaattctttaaagcaagactaacagaaggtctca
ctacagcaaattgcaatgtggcattacaaaaggaggagcataactggcaagagaagagcacaagtgaaaataattatgaactcagacttacatttaaaag
aaaaatatataaatgtcatcacatgttaaggcatctgtaacatatgaaaacttgctaaggtatttgaaacataactttattatgatctaaacgaaaaagg
tatgggaatgacagtaacaagattcaccactgaattttgtgatgtgactgtagcagtcttaacttaagatttgaaacttgagggtagaatatttgcagtc
ccaaacagctaaagacgcaggtccaaaatatgaaggaattattttctgaactgccacattcactccgaagcccattcatctccttctgcatcccaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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