XB-CLONE-4354156
Clone Name: IMAGE:7026557
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783166
[+]
taattaatccccccccccccgagagcccgacacttgtcttgctacaaacagtacctactcacaaaccaacaaccatggttcactggacagctgaagagaa
ggccgccattacctctgtgtggcagaaagtaaacctggagcaagatggccatgaggcactcacaaggctgctggttgtgtacccctggacccagagatac
ttcagcagctttggaaacctcactaatgtcactgctgttgctggaaatgccaaggttcgtgcacatggcaagaaggtcctttcagctgttggtgacgcca
tcacccatcttgacaatgtgaagggcactctccatgacctcagtgaaatccatgccttcaagcttcatgtggatcccgagaacttcaaacgtcttggtga
agtgctggtcattgtcttggcttctaaactgggatcagcttttactcctcaaatccagggagcctgggagaaatttgttgcagttctggttgatgcactc
agccaaggatacaactaaaaatgggcgtacttattgctgtactgaggttatggatactttttatgaacatgttgcaaccattttaaaataaatgagcttg
tataaaacatgtctgtagtttgggttaatatttataccaatgtttttaaaaaaaaaaaaaaaaagggattccctaatttcctaaaaacggcccgcccacc
cccggtgggaaacttgggccgtggggcccaactttgttttattgccccccttttaatgggtttacaaaatataagcgcaaaagcccttccccaattttac
aaagaaaaaaaaaaattttttttttcccggggcaattcaaagatgtggggggtttgggcccccaaccccccctccaagggggaattctttacacaagggg
attcggggaatttggagatataatttaa
3' EST: CF783165
[+]tttttttttttttttaatacatttgtataaatattaaccaaactacagacatgtattatacaagctcatttattttaaattggtgcaacatgttcataaa
agtatccataacctcagtacagcactagtacgccattttagttgtatccttggctgagtgcatcaaccagaactgcaacaaatttctcccaggctccctg
gatttgaggagtaaaagctgatcccagtttagaagccaagacaatgaccagcacttcaccaagacgtttgaagttctcgggatccacatgaagcttgaag
gcatggatttcactgaggtcatggagagtgcccttcacattgtcaagatgggtgatggcgtcaccaacagctgaaaggaccttcttgccatgtgcacgaa
ccttggcatttccagcaacagcagtgacattagtgaggtttccaaagctgctgaagtatctctgggtccaggggtacacaaccagcagccttgtgagtgc
ctcatggccatcttgctccaggtttactttctgccacacagaggtaatggcggccttctcttcagctgtccagtgaaccatggttgttggtttgtgagta
ggtactgtttgtagcaagacaagtgtcgggctctcggggggggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: