XB-CLONE-4354151
Clone Name: IMAGE:7026554
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Gene:
stx5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080503
[+]
CCGGCGCTGACGTCAGCATTTTCCCCGGCAGAAACAGAAAGAGAGAGGGAGTAAGAGAGAAGCGGTGACCGCTGGTCCTGGCCCCATGAACGCTCGTAAA
CGTCTCGGGTCCCGTAACACAGACAACGGAATATACATAGGACCATCCCAGACTCAGGTCCTGGTTCCTGCTTCAGAGTTTCCTGTTGCTCCTCTTCCAG
TTCCCCCAGCTGACATCATGTCCTGTCGGGACCGCACTGCTGAGTTCATTTCTGCTTGCAAATCTCTGCAGGGACGCCAGAATGGTGTACAGTTGTCATC
TCCGACTCTAAGTGCTGTTAAGCAAAGAAGTGAATTCACCCTAATGGCCAAACGCATTGGAAAGGATCTCAGTAACACATTTAGCAAACTGGAGAAACTG
ACAATATTGGCAAAACGAAAATCACTTTTTGATGACAAAGCTGCAGAGATTGAAGAATTGACCTACATAATTAAACAAGATATAGGAAGTCTGAACCAGC
AGATTGCTCAGCTCCAGTCTTTTGTCCGTGCTCGTGGCAGTCAAAGTGGGCGACATCTTCAGACTCATTCAAATACGGTTGTGGTCTCCCTGCAGTCAAA
GCTTGCATCAATGTCTAATGATTTCAAGTCGGTTTTGGAAGTTAGGACAGAGAACCTAAAGCAACAGAGGAGTCGAAGGGAACACTTCTCTCAAGGCCAG
GTGGCACTCCCACTTCATCATAATAGCCTAGGTCCCTCGGTTCTTCTTCAGGATGACTCTCGCAGACAGGGAGATGTGACTATAGAAATGGATTCCAGAG
TTAGTCAGCAGCTGCAGCTTATTGATGAGCAGGACTCATACATCCAAAGTCGAGCAGACACCATGCAGAATATAGAATCTACAATAGTAGAACTGGGATC
CATATTTCAGCAGCTGGCTCATATGGTAAAGGAGCAGGAAGAGACTATACAAAGGATTGATGGAAATGTAGAAGACACTCAGTTAAACGTGGAGGGCGCC
CATCAAGAGATCCTGAAGTATTTCCAATCTGTCACATCGAACCGCTGGCTTATGATCAAGATTTTCCTCATACTTATTGTCTTCTTCATAATCTTTGTAG
TCTTCCTTGCTTAACATCACTCTAGGAAATTGTGAAGTAGTTTCTTCCCCCCAAACACAGCACACAGTGTCCTACTTACCCATGAACCACCCTGCCACTT
AGAAGGGTTACTTTCTGGTTAGTCTGTGGATAAATGTGGGAGGACAGAGGTTAACACACTTACGGATACACTGTGTATATATGTAATGTAAGGATGAGTA
TGCTAAGTGTGATTGAGCAAGGCCATGCATATATGTGTGTGAATAAATCAAATTATTATAGGCATATATATTTTAGACAGTTTGCAGTTAAACACACATG
CACAGTCAGACTTTTGTACTGTGAATGAGGTGTGTGTGTTCTCATGCACACTTTATGTTTTAAGAGTGTGTGTTTACAGTTTTAGGTATGAATACATACA
TATTTACACACACACACACACACACACAATGTGTTTAAAGGCCTTGTATTAATAGCGGTGTGCATGTATGTGTGGTTCCTCTTATTAAGTGTCTTATTGG
GCTTCCTCCACTGAATAAGGCAAGTGGAACCTGCTTCTTGTGGGGAGGGAGATAAGTGGCTTTGTCCAAGGCTGTATTAGTGAGAGCTGACAATATGTAC
AGGCCAGGCACGCTTGTTTTAATCTCTTGTAAACAATGTAATATTCTGTTTTTAAATTATTCTTGGAAAAAAAAAAAAAAAA
5' EST: CF783169
[+]taattaatcccccccccccccccggcgctgacgtcagcattttccccggcagaaacagaaagagagagggagtaagagagaagcggtgaccgctggtcct
ggccccatgaacgctcgtaaacgtctcgggtcccgtaacacagacaacggaatatacataggaccatcccagactcaggtcctggttcctgcttcagagt
ttcctgttgctcctcttccagttcccccagctgacatcatgtcctgtcgggaccgcactgctgagttcatttctgcttgcaaatctctgcagggacgcca
gaatggtgtacagttgtcatctccgactctaagtgctgttaagcaaagaagtgaattcaccctaatggccaaacgcattggaaaggatctcagtaacaca
tttagcaaactggagaaactgacaatattggcaaaacgaaaatcactttttgatgacaaagctgcagagattggaaaattgacctacttaattaaacaag
atataggaagtctgaaccagcagattgctcagctccagtcttttgtccgtgctcgtgggcattcaaagggggggaaatctttcaaacttatttaaaatac
gggttgggggtctcccctgcaggtcagaaggtttggattcgaaaggccccaaatgaatttcccaagtcggtgtttttgggaaatttttagggaacaaaaa
taaacctctaaaaggctaagcgaaagagaattcttaaaggggggaaacccttttcttcttacatggggcccagtgttggtgttcttccccaccttttttt
ccattaataacccccttgggtctgactgtgggttttttctattcccggatgtaaaatctctgccataaaaggtggagtttggtttgtttttatgaacatg
gggttctcccataagtttatccctaaaaacgttcgctgttttttatttataaaaatgactttatttattttcccaaaggtgctagctgtgaacccctgtt
ccgcctaattccaaatcttctctttattgagcaaactgggggttccat
3' EST: CF783168
[+]tttttttttttttttccaagaataatttaaaaacagaatattacattgtttacaagagattaaaacaagcgtgcctggcctgtacatattgtcagctctc
actaatacagccttggacaaagccacttatctccctccccacaagaagcaggttccacttgccttattcagtggaggaagcccaataagacacttaataa
gaggaaccacacatacatgcacaccgctattaatacaaggcctttaaacacattgtgtgtgtgtgtgtgtgtgtgtaaatatgtatgtattcatacctaa
aactgtaaacacacactcttaaaacataaagtgtgcatgagaacacacacacctcattcacagtacaaaagtctgactgtgcatgtgtgtttaactgcaa
actgtctaaaatatatatgcctataataatttgatttattcacacacatatatgcatggccttgctcaatcacacttagcatactcatccttacattaca
tatatacacagtgtatccgtaagtgtgttaacctctgtcctcccacatttatccacagactaaccagaaagtaacccttctaagtggcagggtggttcat
gggtaagtaggacactgtgtgctgtgtttggggggaagaaactacttcacaatttcctagagtgatgttaagccaggaagactaccaagattttgaaaaa
agacaataagtatgagggaaaatctttgatcataacccagcgggttcgatgtggacagattggaaaatacttccaggatctcttggatggggcgcccttc
cagttttaaactgaaatgccctctaacattt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: