XB-CLONE-4354149
Clone Name: IMAGE:7026553
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Gene:
actb
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723026
[+]
taattaatccccccccccccccctcagtgacccgcccgcacagaaacaggacagcgtgtgtgtcgtactctcagattacaatggacgacgatattgctgc
actggtcgttgacaatggttctggtatgtgcaaagccggctttgcaggtgatgatgcaccccgtgctgttttcccatccattgttggtcgcccaagacat
cagggtgtcatggttggaatgggacagaaagatagctacgtaggagacgaagctaaaagcaaaagaggtattcttacactcaaatatcctattgagcatg
gcattgtcactaactgggatgatatggagaagatctggcatcataccttctacaatgaactcagagtggccccagaggaacacccagtgctgctgacaga
agcacccttgaatcccaaagctaaccgggagaaaatgacgcagatcatgtttgagacctttaacactccagctatgtatgttgccatccaagctgtgttg
tccctgtatgcatctggtcgtaccactggtattgtcatggactcaggtgatggtgtaacacacactgtgccaatatatgaaggttatgctctaccccatg
ccattctgcgtctggacttggctggacgtgacctgacagactacctcatgaaaatcctcactgaaagggggtacagcttccccaccacagctgaaagaga
aattgtttcttgatatcaaggaaaaattgtgcttatgttgcctctggacttttgaacaaggagaatgggccccagcctggctttcttcctttcatcaatt
gggaaaaaaaaaatttattgaaccctggcctcggatggggacaaagttttatccccccccttttggtgtaaaattgaagcgggggtttttaaaaaatggt
ctcccaaaaaaagaccaactctcctttttcccagacccccctttttcttttttccccctgtgggggagat
3' EST: CF783170
[+]tttttttttttttttggtgagaaacggatgtgaaacaacttggttttatttttttcagttaggttacaggtgacccgtcacatcttatgtgatcaatgat
aatgtaaaccttccaggtgttagagaatgatgcccttttttgggttaaacaagttacaagtgttcaaggcagaagatacagagacatgtttgggtacata
gctgcacaagaaaaaaaaaacaaaaaaacaaaaaaaatcaaaaaacacaatagtatcaccatgatttctgtatgagcttcccaccaagctgggcaacatt
gaaagggtagcttcctcaatacaatactattgactgaataaaatcataatgtcctctgcattgatgaccatacagtgctatttgttcattccagttatca
aatctgagccaagcgccaatgaaagaaaagccatgccaatgttatcttcctgcacaggtacatttgttcccgttgaaagggtccgtccttttaaaagcct
ttaccatgctctattgaaggcccggactcatcatacttctggcttgctgaatcacaccttcctcggaaggtggaacagggaaaccaaaattggaaccttc
caattccaacaacagctttttaccctccagggggaaacacatcaccccggatttttccacgggtgcctggggagcctatggcagacttattttttttcct
ccctttctattccccaattttcaaagggaaaatttggggggttctcccccttataaagaaacaggcactttggtattaaaagggttccctttacggaata
atcttatattcaccaatttccccacaatttacaaattcatacaacccgggttttccttcaaaatactcctcacggaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: