XB-CLONE-4354146
Clone Name: IMAGE:7026552
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Gene:
actg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783172
[+]
taattaatccccccccccccccgtcattcagccggtggcagcggacttgacagctacttcccgctctttgtaacaacttgccagctttgcatttctcctc
ttaaggaaacctccagcaccaagaaataaaatggatgaggaaattgcagcactcgtcgttgacaatggctctggaatgtgcaaagccggctttgtatgga
gatgatgctccccgtgctgtgttcccctctattgttggacgccctagacaccaaggtgtaatggttggtatgggccaaaaggacagctatgtaggtgatg
aagcacaaagcaagaggggtatcctaaccctgaaggatccaattgaacccggaattgtgtcaaactgggatgaaatggagaagattgggactctcaccta
ctacagtggagtttcgtgtgagacccatgaaaaggataccaatgcctgcattaacagaaagctaccgcagtaacacgtatatgggttatgtgaggaagtg
tatgggattccttattcaatggattgggaagaattttttcaaatcgcgcgcgaaggagaaaggcaattcgtgcggcaagatttctcaaacccataggacc
aatgacactaattgatatattgcttcatctgtgggtaacatattgaggttaccctcgcgtctattacacgtttggtattgtgatacaaactcgtgccgtg
aagacgtaagtacgaggatctcttaacatctgtcgagacgaggatcaatatattccctttattaatatataataggaaagataatgantatatataatga
catctttatggagccgccgtcgaagaggtggaaagttatcccatcattttgtagacgtatataaagatggttatagaggagtgagaatagcggagggaca
cgagagagaaagatgatgtgtaagctaggtatttacataaaaagacgaatgtatccatatatacttaagctatgcgcaattacacaacangttgatcgac
ctcattactagtgatataatttataggacatgatagaattgatatattgtgatgtatgagagaagaaaacaattataatgctattatctgacaatctcac
aaaaatgataatatagcgaaaaacaagggagggcacgagggancgtgcacaaacacacgagcgcactttcaaacaaaaatcagaagaaatgggtgttact
taagaatgtattcaagtcacactgcgtaagtaatatgaatataaacataaagttaaatctgcntattttgtgacatttattaatccaatacacgtgtacc
an
3' EST: CF783171
[+]tttttttttatttttatttactgcagcgctttattttatccttacagaacggtgttttggaagttacaattctcatctggatgacatacaaattttggtt
ttctacgttgtgagaacattaaggcaacatacaaaaaaatttacataaattaacttggatgcataaatttacaggtgttaactgcaacatttgtaacttt
cttccgacccattttgaatgttgggaatacaacctgggttaagaaaaagactggttcttgggcctgagtgtcaatttccaaccttactataaactggaaa
gatagaagagattccttcatgaactgaccagcctccgaaatgaaacaatggagtattaagcgcttaactgtgtgtggaagtctaaacctcaaataccctg
tacacatctccaaaactcgacttctccataaaccgcccctgtaacctataagagaagtggcattcgccaatactgtcccaaatcggtagccttatcaaga
aaaggctttctttccacgtaaaggcataaacctaaatttcaccccatactgtttgcccgcctcggccctataaaagacgcgcttcacggctaaaaaatgt
taacatttgataccccagggaatgaattttgaaaaaaacaaaccaccaggtttggcattatacaaataccccccaaaaactgaaccaagatgagatcctt
caatttcccaagcgaaaacatcaaccttgggaaaattggccctccttctttccagtttccagaaaaggcttccttagacggctttttaacagtttttggc
caagggaacaattcataactttttgacacaaacacacgcatccactatattggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: