XB-CLONE-4354136
Clone Name: IMAGE:7026547
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Gene:
ap3m2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723028 [+]
taattaatcccccccccccccccgctggttacgtgctttttaatggtgccggaacatcctgtgtgaggcaaacagaagaaactctccgcaccttacatct
gccttgcccagcccatcatgatccacagcctgtttttgattaacccttcgggggacatcttcctagagaagcactggaaaagtgtggtatcccgctctgt
ttgtgactacttctttgaagcccaggaaagggcatcagaggcagagaatgtgcccccaatcattcagacccctcaccactaccttctcagcgtctacagg
cacggcatcttttttgtggctgtcattcagacagaagtgcccccgcttttcgccattgagttccttcaccgcgtggtggacacattccaggattattttg
gctcctgctctgaggctgtgattaaggaaaacgtggtaattgtatatgaggttcttgaggaaatgctggataatgggtttccctctgctactgaattcaa
cattcctgaaggaagctcatcaagcctccaaccattcttacgcagtgtggtccaaccccatcaaaaggcagtttccaaatgttgggggaaccaactttcc
ctagtggaagatggctctaccgtggggttcccttggaatatagggacacaggaaattaataatacccccccgattagattgaaaggcgcttatttttttt
ggacggttttatccaaagagaaaaattggatgcgccaactcctattggattataatttcaggggtatgccatctttgtttaaaacccaacaaaaattttt
ccggggggggctggaaatcccaaccaccttgttttataaataactttcgtcctcggggaggccccccaaaaccagaaacccgggggactcttcccggaaa
aatcaattcatttctcctctagtcatggacatgttaagatttttttccaagtcttgacggggtatgattatttacaaacatgtcgggtggtgaggccaga
aagaggggtagtcctatttcccatttcattatctcgccg
3' EST: CF783178 [+]tttttttttttttttgctcacacattttacagatttatttgtaataattatcttagtaaaacaccattttggaagatatggtaacatttctgcatataac
agaatagccttcacaaataacaatgcataattcctatccaaactgccagtggttggcaggggatgatgggagctcttgacgagcaacaggtgcagaagtc
tggcatccttgctcttcctctgcaccctctgcacatgtaatgtactgaatgggctcctaaaacacccatcacacagatgcacacaagaattctcccagaa
caccctttataacagggaatacacacacatataacccccccaactatgctgtccaacactcccagaagacacactgagtagtcacactctctctcatctc
atctgcatatagagggggaagttgtcacaagctgaataggggggtacttcaagacatagctcaaacttccccccttcatgttctgacttggaacttgcca
gctttggtcacgcacctgatgccttcgaatgggttgtatcttcatttccaaacatgtccaccctgttttccttcctatctgctttgcgcttactgttggt
attctttaccgccttgttccacggtggctgtttttcttcttctttccagagcaactattccttaatttacccttgttcccctcctacgatttgggaacct
tccagagcgatataatttccccttaaccctttagtacacatttccttgttttttttgcgcttaacgaacatctaaattttttatgttaaaaccggtttca
ctcccactttttttcactccactcccataaggactcttttgaacc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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