XB-CLONE-4354133
Clone Name: IMAGE:7026546
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Gene:
khdrbs1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783180
[+]
taattaatcccccccccccccggatttttcttatcgacaccagcactgagcgtattgccgaccagtaacaccgaaccgagcaacatggagaccgagacca
aatacttacccgaactgatggctgagaaggacagcctggacccatctttcacgcacgctatgagcctcctgggcaaggaaattgaaaggctgaagaaggg
aggtgatgccaagaaagatgaagaagatacttatctggacttattttcccataaaaacatgaagttaaaagagcgcattttgataccagtcaagctgtat
cccaagtttaactttgttggaaagatccttggaccacagggaaacaccatcaaaaggttacaggaggagactggagcaaaaatctctgttcttggcaagg
gatcaatgagagataaggcaaaggaagaagagctgcggaaaggaggggaccccaaatacagccatctgaatatggaccttcatgtgttcatagaggtctt
tggaccaccatgtgaatcttatacacgtatggcacatgcaatggaagaagtcaagaagttcttggttccggacatgatggatgatatctgtcaggaacag
tttatggagctatcttatctttatggagcaccaccagagcaaagcagaggtggaacaagaggtggaccatccaggggcagaggggggcccccacctgctg
cagcttccttcctttaaaaggcaggggctgggccctcttccgcccctcttgtttccaaggaggggccccccgggaagtgggaagcctttatccgcgtggg
tggccttatgttggaagcccggccctggtttccccccctttccatcctgtgctagccccgaagggggggccccaccccccctgggggaaataaaaccacc
gcgggggaggggctttggccctaatttcacctcaaaagaagtgtccctcctccccn
3' EST: CF783179
[+]tttttttttttttttattgtcctttcaaaggaatttgggaaaagggagctatatgagggaggtcacagagcacagagaatttacttgtgtattgtgggtt
aaggaacaggaagtcccgaacacaagacatcggtaaacatggtttggatgaaaagatgttcaaaagcgcccataaggatgttctctgtatgcaccgccct
tcaaaggacgtgaagagggaaccttaagtgaaggtcgtgatccattccaattatcttgtccataactctcatacgattcaggaccctctccatgaccata
atcataatattcagtctctccttggctttgactgtaatatccctcataaccttcatagcttggctctgcataggagtcttcataaccatattcatcatat
gtttcaggtgcaggaggtggaagagagattctctggatagcagcaccacgggctcttccagcgggtgcacctcggctagcagaggaagggggtacagggc
ggctcacactagcaccacgcgatatggctccacgtccgggggcacctcttggaacaagagggcgaagaggcccagccctgcctctagaagaaggagctgc
agncaggtggtggaccccctctgcccctggatggtccacctcttgttccacctctgctttgctctggtggtgctccattaagataagatagctccataaa
ctgttcctgacagatatcatccatcatgtccggaaccaagaacttcttgacttcttccattgcatgtgccatacgtgtataagatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: