XB-CLONE-4354131
Clone Name: IMAGE:7026545
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Gene:
rbbp7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723029
[+]
taattaatccccccccccccccccctttgtgagtgtttggcgcgggaagctgcagcaggaccggcacaagcgcggaacactgggtttttagtcaccgcga
ggccggcggcccttgcatctgcacaggagtagagaagatggcgaataaagagatgtttgaagatacagtggaagaacgtgtcatcaatgaagagtataag
atatggaaaaaaaataccccatttctgtatgacctggttatgacccatgcacttgaatggccaagtctaacagttcagtggctcccggatgtgactagac
caaaaggaaaggactatgcgctgcactggttggtcttaagtactcacacttcagatgaacaaaatcaccttgtagtggctcgtgtccagattcccaatga
ccatgcccagtttgatgcctcacattatgatagtgagaaaggatagtttcggtggccttcggttctgttaatgggcaaaaattgaaacggaaattaaaat
taacccttgaagggagagggtaaaccggcggcccgttaacagtgccccaaaaatcgatgttttgaatgtgcaaactgaaaaaccccctgtcccggcaaga
atgtgcctcgggttttttttatatataaccaaaagaattccctttatctctaaccatggaacacccaagaagggaaacaaatgagcaagttcactttaga
actttcaagaaacatttaagtaataaacaacttacgacacaggaaacaggctattcttgtgtcacttaattacatgtgggaaactttccatgtacntatt
ttgaattcgggcgaccacgtctatttctcctaaaataggtccgcctattagaatgtgcccttcatcttcgaggcgtgtttatggtctattctatgcgcgt
gcaactttcatgtcgcacctcagaaggtctattttaaaatacaaaaagagcgcatatacgtggaaatacttctagaaaattctctaagaaaagcagcata
taatatatttatctatgaangtttctctctatctaaatagtatttctatcttccctccataaaataggaaagcgtgatggacgntcgtcgaacatcactc
ctcttnctcccggn
3' EST: CF783181
[+]ttttttttttttttattccatcagccgcttcagcgagtcccccaggtatccaatacaaagagcaagaatattacaatacaatttacaaagaaaatgtcaa
tacatgtactcagaattgcaaattaacttacacaaacactaaccacgtcacaaatgtggcagcatctgcaatagtgagtgtgaataagaaaaaggaaaaa
gatctatgatccttgagcttctagctcagaagctggtatgtcaggttcttcatcattgtaaatattttcagccatctgccatatttgcatgatgttatct
tcagacactgaacatatcacccatggttcatttgggttccagctgaagtcagagatttttgctgtgtgccctccatgtataaacaaaagctcaggaggtc
catcctcagcatcttctgctgactgctcttctccaattttacttaaatcccagacattaagtcttcgatctgtaccgctggaagctaaaatagtttcatt
atgaggagaccagtgaacctggaaaatttcatccttatgagattcgaaagaaaggagcttcagctttaaattcctgagattccaaagaactactgtcttt
attcgcaaaacctgtgggccaaaatgacctctcctttatgggctttgaaggacaagtattttgcccctccccagtcttgcgcccttccactgaaaggggc
ggggtttaaaaaatgggtatcttgacccttttgttcccccaattaaaaaaactttttggaatcatcctggagaaaaaattctaaaaaaaggcaattttcg
caaaaatggtttcccctacccaaaacccctttattatatggtag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: